2. Academic Validation
  3. Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity

Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity

  • Biochem Biophys Res Commun. 2017 Jan 29;483(1):509-515. doi: 10.1016/j.bbrc.2016.12.114.
Yang Ke 1,  Tianhao Bao 2,  Xuesong Wu 3,  Haoran Tang 3,  Yan Wang 4,  Jiayun Ge 1,  Bimang Fu 1,  Xu Meng 5,  Li Chen 6,  Cheng Zhang 1,  Yuqi Tan 1,  Haotian Chen 1,  Zhitang Guo 1,  Fan Ni 1,  Xuefen Lei 7,  Zhitian Shi 1,  Dong Wei 1,  Lin Wang 8
Affiliations

Affiliations

  • 1 Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • 2 Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China; Mental Health Center, Kunming Medical University, Kunming, China.
  • 3 Department of Gastroenterological Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • 4 Department of Pathology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • 5 Intensive Care Unit, The First Hospital of Kunming, Kunming, China.
  • 6 Department of Pediatric Surgery, The Kunming Children's Hospital, Kunming, China.
  • 7 Department of Oncology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • 8 Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. Electronic address: [email protected].
PMID: 27998773 DOI: 10.1016/j.bbrc.2016.12.114
Abstract

Scutellarin is an active flavone from Erigeron breviscapine (vant) Hand Mass. This study aimed to investigate the potential role of scutellarin in migration and invasion of human hepatocellular carcinoma (HCC) cells and its possible mechanism. In comparison with the vehicle-treated controls, treatment with scutellarin (50 mg/kg/day) for 35 days significantly mitigated the lung and intrahepatic metastasis of HCC tumors in vivo. Scutellarin treatment significantly reduced HepG2 cell viability in a dose-dependent manner, and inhibited migration and invasion of HCC cells in vitro. Scutellarin treatment significantly reduced STAT3 and Girders of actin filaments (Girdin) expression, STAT3 and Akt phosphorylation in HCC cells. Introduction of STAT3 overexpression restored the scutellarin-downregulated Girdin expression, Akt activation, migration and invasion of HCC cells. Furthermore, induction of Girdin overexpression completely abrogated the inhibition of scutellarin on the Akt phosphorylation, migration and invasion of HCC cells. Scutellarin can inhibit HCC cell metastasis in vivo, and migration and invasion in vitro by down-regulating the STAT3/Girdin/Akt signaling.

Keywords

Akt; Girdin; Hepatocellular carcinoma; Invasion; STAT3; Scutellarin.

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