2. Academic Validation
  3. Anti-inflammatory activity of compounds from the rhizome of Cnidium officinale

Anti-inflammatory activity of compounds from the rhizome of Cnidium officinale

  • Arch Pharm Res. 2018 Oct;41(10):977-985. doi: 10.1007/s12272-018-1048-9.
Huynh Nguyen Khanh Tran 1,  Thao Quyen Cao 1,  Jeong Ah Kim 2,  Ui Joung Youn 3,  Sanghee Kim 3,  Mi Hee Woo 1,  Byung Sun Min 4
Affiliations

Affiliations

  • 1 College of Pharmacy, Drug Research and Development Center, Daegu Catholic University, Gyeongbuk, 38430, Republic of Korea.
  • 2 College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea. [email protected].
  • 3 Division of Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea.
  • 4 College of Pharmacy, Drug Research and Development Center, Daegu Catholic University, Gyeongbuk, 38430, Republic of Korea. [email protected].
PMID: 29961195 DOI: 10.1007/s12272-018-1048-9
Abstract

Five new compounds, 9,3'-dimethoxyhierochin A (1), 6-oxo-trans-neocnidilide (2), (±)-(3E)-trans-6-hydroxy-7-methoxydihydroligustilide (3), (±)-cnidiumin (4), and 6-(1-oxopentyl)-salicylic acid methyl ester (5), together with twenty known compounds (6-25), were isolated from the rhizome of Cnidium officinale. The chemical structures of new compounds were established by NMR spectroscopic techniques, mass spectrometry, Mosher's method, and CD spectrum. Their anti-inflammatory activities were evaluated against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW 264.7 cells. Compounds 7, 13, and 14 showed inhibitory effects with IC50 values of 5.1, 24.5, and 27.8 μM, respectively. In addition, compounds 7, 13, and 14 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression and cyclooxygenase-2 (COX-2) protein in a concentration-dependent manner.

Keywords

Anti-inflammatory; Cnidium officinale; NO inhibition; Umbelliferae.

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