2. Academic Validation
  3. Glycosylation of Methoxylated Flavonoids in the Cultures of Isaria fumosorosea KCH J2

Glycosylation of Methoxylated Flavonoids in the Cultures of Isaria fumosorosea KCH J2

  • Molecules. 2018 Oct 9;23(10):2578. doi: 10.3390/molecules23102578.
Monika Dymarska 1,  Tomasz Janeczko 2,  Edyta Kostrzewa-Susłow 3
Affiliations

Affiliations

  • 1 Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, Wrocław 50-375, Poland. [email protected].
  • 2 Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, Wrocław 50-375, Poland. [email protected].
  • 3 Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, Wrocław 50-375, Poland. [email protected].
PMID: 30304815 DOI: 10.3390/molecules23102578
Abstract

Flavonoids are widely described plant secondary metabolites with high and diverse pro-health properties. In nature, they occur mostly in the form of glycosides. Our research showed that an excellent way to obtain the sugar derivatives of Flavonoids is through biotransformations with the use of entomopathogenic filamentous fungi as biocatalysts. In the current paper, we described the biotransformations of five methoxylated flavonoid compounds (2'-methoxyflavanone, 3'-methoxyflavanone, 4'-methoxyflavanone, 6-methoxyflavanone, and 6-methoxyflavone) in cultures of Isaria fumosorosea KCH J2. As a result, we obtained twelve new flavonoid 4-O-methylglucopyranosides. The products were purified with methods that enabled the reduction of the consumption of organic solvents (preparative TLC and flash chromatography). The structures of the products were confirmed with spectroscopic methods (NMR: ¹H, 13C, HSQC, HMBC, COSY). The compounds obtained by us expand the library of available flavonoid derivatives and can be used in biological research.

Keywords

4-O-methylglucopyranoside; Isaria; biotransformations; flavonoid glycosides; flavonoids; fungi; methoxyflavanone; methoxyflavone.

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