2. Academic Validation
  3. Secondary Metabolites and PI3K Inhibitory Activity of Colletotrichum gloeosporioides, a Fungal Endophyte of Uncaria rhynchophylla

Secondary Metabolites and PI3K Inhibitory Activity of Colletotrichum gloeosporioides, a Fungal Endophyte of Uncaria rhynchophylla

  • Curr Microbiol. 2019 Jul;76(7):904-908. doi: 10.1007/s00284-019-01707-7.
Zhong-Duo Yang 1,  Zhi-Jie Li 2,  Jun-Wen Zhao 2,  Jian-Hui Sun 2,  Li-Jun Yang 2,  Zong-Mei Shu 2 3
Affiliations

Affiliations

  • 1 School of Life Science and Engineering, Lanzhou University of Technology, 287 Lan Gong Pin Road, Qi Li He Block, Lanzhou, 730050, Gansu Province, People's Republic of China. [email protected].
  • 2 School of Life Science and Engineering, Lanzhou University of Technology, 287 Lan Gong Pin Road, Qi Li He Block, Lanzhou, 730050, Gansu Province, People's Republic of China.
  • 3 The Provincial Education Key Laboratory of Screening, Evaluation and Advanced Processing of Traditional Chinese Medicine and Tibetan Medicine, School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, People's Republic of China.
PMID: 31104137 DOI: 10.1007/s00284-019-01707-7
Abstract

In the present study, nine compounds (1-9) were isolated from Colletotrichum gloeosporioides (an endophytic fungus from Uncaria rhynchophylla) which was cultured in wheat bran medium. Their structures were elucidated as 4-Epi-14-hydroxy-10, 23-dihydro-24, 25-dehydroaflavinine (1), 10, 23-Dihydro-24,25 -dehydro-21-oxoaflavinine (2), Ergosterol (3), Ergosterol peroxide (4), Mellein (5), 4, 5-dihydroblumenol A (6), Colletotrichine A (7), Cyclo(L-leucyl-L-leucyl) (8), and Brevianamide F (9) based on NMR spectral data, as well as comparing with previous literature data. This is the first report about the isolation of compounds 1-2, 6, and 8-9 from Colletotrichum genus. All compounds were tested for their phosphoinositide 3-kinase (PI3Kα) inhibitory activity. Compounds 8 and 9 showed potent PI3K α inhibitory activity with IC50 values of 38.1 and 4.8 µM, respectively, while the other compounds showed very weak activity at a concentration of 20 µg/mL.

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