2. Academic Validation
  3. Docetaxel-loaded PAMAM-based poly (γ-benzyl-l-glutamate)-b-d-α-tocopheryl polyethylene glycol 1000 succinate nanoparticles in human breast cancer and human cervical cancer therapy

Docetaxel-loaded PAMAM-based poly (γ-benzyl-l-glutamate)-b-d-α-tocopheryl polyethylene glycol 1000 succinate nanoparticles in human breast cancer and human cervical cancer therapy

  • J Microencapsul. 2019 Sep;36(6):552-565. doi: 10.1080/02652048.2019.1654002.
Yingting Wang 1,  Along Zuo 2,  Xiaoyan Huang 1,  Ying Ying 1,  Xinsheng Shu 1,  Xianxiong Chen 1,  Yatao Yang 3,  Junxian Ma 3,  Guimiao Lin 1,  Xiaomei Wang 1,  Lin Mei 4 5,  Gan Liu 4 5,  Yingying Zhao 1
Affiliations

Affiliations

  • 1 Department of Physiology, School of Medicine, Shenzhen University , Shenzhen , China.
  • 2 Key Laboratory for Natural Resource of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University , Yanji , China.
  • 3 School of Information Engineering, Shenzhen University , Shenzhen , China.
  • 4 School of Life Sciences, Tsinghua University , Beijing , People's Republic of China.
  • 5 The Shenzhen Key Laboratory of Gene and Antibody Therapy, Center for Biotechnology and Biomedicine and Division of Life and Health Sciences, Graduate School at Shenzhen, Tsinghua University , Shenzhen , China.
PMID: 31403342 DOI: 10.1080/02652048.2019.1654002
Abstract

Taxane-based chemotherapy-loaded drug delivery systems have great potential for Cancer treatment. The docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate)-b-d-α-tocopheryl polyethylene glycol 1000 succinate (PAM-PBLG-b-TPGS) nanoparticles and the docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate) (PAM-PBLG) nanoparticles were designed using a modified nanoprecipitation method. The particle size, encapsulation efficiency (EE), and in vitro release characteristics of the nanoparticles were tested. The effects of the two nanoparticles on the cellular uptake and cell viability on human cervical Cancer cell line Hela and the human breast Cancer cell line MCF-7 were compared. Furthermore, their antitumor efficiency was evaluated through in vivo tumour growth experiment in comparison with free DTX. PAM-PBLG-b-TPGS nanoparticles displayed high EE, smaller diameter, and a nice releasing profile. Besides, based on the high EE and 'self-controlled' drug release of the DTX-loaded PAM-PBLG-b-TPGS nanoparticles, they exhibited stronger cytotoxicity (lower survival rate) and higher uptake rate than DTX-loaded PAM-PBLG nanoparticles in Hela cells and MCF-7 cells. Furthermore, compared with DTX-loaded PAM-PBLG nanoparticles and free DTX, DTX-loaded PAM-PBLG-b-TPGS nanoparticles produced a potent anti-tumour effect. Thus, the DTX-loaded PAM-PBLG-b-TPGS nanoparticles provide a novel attractive nanocarrier for the DTX delivery of chemotherapy to human breast Cancer cells and human cervical Cancer cells.

Keywords

Docetaxel; cancer chemotherapy; drug delivery; nanoparticles.

Figures
Products