2. Academic Validation
  3. Multimerization through Pegylation Improves Pharmacokinetic Properties of scFv Fragments of GD2-Specific Antibodies

Multimerization through Pegylation Improves Pharmacokinetic Properties of scFv Fragments of GD2-Specific Antibodies

  • Molecules. 2019 Oct 24;24(21):3835. doi: 10.3390/molecules24213835.
Irina V Kholodenko 1,  Daniel V Kalinovsky 2,  Elena V Svirshchevskaya 3,  Igor I Doronin 4 5,  Maria V Konovalova 6,  Alexey V Kibardin 7,  Tatyana V Shamanskaya 8,  Sergey S Larin 9,  Sergey M Deyev 10 11,  Roman V Kholodenko 12 13
Affiliations

Affiliations

  • 1 Orekhovich Institute of Biomedical Chemistry, 10, Pogodinskaya St., Moscow 119121, Russia. [email protected].
  • 2 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10, Miklukho-Maklaya St., Moscow 117997, Russia. [email protected].
  • 3 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10, Miklukho-Maklaya St., Moscow 117997, Russia. [email protected].
  • 4 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10, Miklukho-Maklaya St., Moscow 117997, Russia. [email protected].
  • 5 Real Target LLC, Miklukho-Maklaya St., 16/10, Moscow 117997, Russia. [email protected].
  • 6 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10, Miklukho-Maklaya St., Moscow 117997, Russia. [email protected].
  • 7 D. Rogachev Federal Research Center of Pediatric Hematology, Oncology and Immunology, 1, Samory Mashela St., Moscow 117997, Russia. [email protected].
  • 8 D. Rogachev Federal Research Center of Pediatric Hematology, Oncology and Immunology, 1, Samory Mashela St., Moscow 117997, Russia. [email protected].
  • 9 D. Rogachev Federal Research Center of Pediatric Hematology, Oncology and Immunology, 1, Samory Mashela St., Moscow 117997, Russia. [email protected].
  • 10 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10, Miklukho-Maklaya St., Moscow 117997, Russia. [email protected].
  • 11 Sechenov First Moscow State Medical University, 8-2, Trubetskaya St., Moscow 119992, Russia. [email protected].
  • 12 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10, Miklukho-Maklaya St., Moscow 117997, Russia. [email protected].
  • 13 Real Target LLC, Miklukho-Maklaya St., 16/10, Moscow 117997, Russia. [email protected].
PMID: 31653037 DOI: 10.3390/molecules24213835
Abstract

Antigen-binding fragments of Antibodies specific to the tumor-associated ganglioside GD2 are well poised to play a substantial role in modern GD2-targeted Cancer therapies, however, rapid elimination from the body and reduced affinity compared to full-length Antibodies limit their therapeutic potential. In this study, scFv fragments of GD2-specific Antibodies 14.18 were produced in a mammalian expression system that specifically bind to ganglioside GD2, followed by site-directed pegylation to generate mono-, di-, and tetra-scFv fragments. Fractionated pegylated dimers and tetramers of scFv fragments showed significant increase of the binding to GD2 which was not accompanied by cross-reactivity with other gangliosides. Pegylated multimeric di-scFvs and tetra-scFvs exhibited cytotoxic effects in GD2-positive tumor cells, while their circulation time in blood significantly increased compared with monomeric antibody fragments. We also demonstrated a more efficient tumor uptake of the multimers in a syngeneic GD2-positive mouse Cancer model. The findings of this study provide the rationale for improving therapeutic characteristics of GD2-specific antibody fragments by multimerization and propose a strategy to generate such molecules. On the basis of multimeric antibody fragments, bispecific Antibodies and conjugates with cytotoxic drugs or radioactive isotopes may be developed that will possess improved pharmacokinetic and pharmacodynamic properties.

Keywords

antibody fragments; cancer; ganglioside GD2; immunotherapy; multimerization; neuroblastoma; pegylation.

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