2. Academic Validation
  3. Generation and characterization of a cell line from an intraductal tubulopapillary neoplasm of the pancreas

Generation and characterization of a cell line from an intraductal tubulopapillary neoplasm of the pancreas

  • Lab Invest. 2020 Jul;100(7):1003-1013. doi: 10.1038/s41374-020-0372-0.
Matthäus Felsenstein  # 1 2,  Maria A Trujillo  # 1,  Bo Huang  # 1,  Neha Nanda 1,  Zhengdong Jiang 1 3,  Yea Ji Jeong 1,  Michael Pflüger 1 2,  Michael G Goggins 1 4 5,  Ralph H Hruban 1 4,  Elizabeth D Thompson 1,  Christopher M Heaphy 1 4,  Nicholas J Roberts 6 7,  Laura D Wood 8 9
Affiliations

Affiliations

  • 1 Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
  • 2 Department of Surgery, Charité Universitätsmedizin Berlin, 10117, Berlin, Germany.
  • 3 Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
  • 4 Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
  • 5 Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
  • 6 Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA. [email protected].
  • 7 Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA. [email protected].
  • 8 Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA. [email protected].
  • 9 Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA. [email protected].
  • # Contributed equally.
PMID: 32005909 DOI: 10.1038/s41374-020-0372-0
Abstract

Intraductal tubulopapillary neoplasm (ITPN) is a distinct precancerous lesion in the pancreas with unique clinical and molecular features. Although in vitro studies in two-dimensional culture have led to numerous important insights in pancreatic Cancer, such models are currently lacking for precancerous lesions. In this study, we report the generation and characterization of a cell line from a human pancreatic ITPN. Neoplastic cells were initially cultured in a three-dimensional organoid system, followed by transfer to two-dimensional culture. RNA sequencing revealed a gene expression profile consistent with pancreatic ductal origin, and whole genome sequencing identified many somatic mutations (including in genes involved in DNA repair and Wnt signaling) and structural rearrangements. In vitro characterization of the tumorigenic potential demonstrated a phenotype between that of normal pancreatic ductal cells and Cancer cell lines. This cell line represents a valuable resource for interrogation of unique ITPN biology, as well as precancerous pancreatic lesions more generally.

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