Characterization of an i.p. D-galactose-induced cataract model in rats

Cataracts have been identified as a main cause of global visual impairment and blindness; in addition, diabetic and aging cataracts are the most common types. The aim of this project was to develop a suitable animal model and investigate the key points of the mechanisms by which intraperitoneal (i.p.) injection of D-galactose forms cataracts. We optimized a method to investigate the safest and effective method and dosage; rats in Group H were treated with 50% D-galactose 15 g/kg i.p. twice daily based on the 11 different treatment methods. The simple oral group showed considerable differences in the same observed time, while the i.p. group showed relatively uniform cataracts due to intake of the same dose of D-galactose. The data suggest that i.p. injection of galactose is a relatively more successful and stable cataract-inducing method with a low mortality rate. Based on this model, we found that Na⁺/K⁺ ratios had important relevance for galactose cataract formation, and we used scanning electron microscopy (SEM), inductively coupled plasma mass spectrometry (ICP-MS), enzyme-linked immunosorbent assay (ELISA) and immunofluorescence examinations to test and verify this.

Cataract; D-galactose; Element of lens; Na(+)/K(+) ratio; Rat.