2. Academic Validation
  3. Dictamnine delivered by PLGA nanocarriers ameliorated inflammation in an oxazolone-induced dermatitis mouse model

Dictamnine delivered by PLGA nanocarriers ameliorated inflammation in an oxazolone-induced dermatitis mouse model

  • J Control Release. 2021 Jan 10:329:731-742. doi: 10.1016/j.jconrel.2020.10.007.
Chin-Yu Lin 1,  Yun-Ting Hsieh 2,  Long Yi Chan 3,  Ting-Ya Yang 2,  Tomoji Maeda 4,  Tsong-Min Chang 5,  Huey-Chun Huang 6
Affiliations

Affiliations

  • 1 Institute of New Drug Development, College of Medicine, China Medical University, Taichung 40402, Taiwan; Tsuzuki Institute for Traditional Medicine, Collage of Pharmacy, China Medical University, Taichung, 40402, Taiwan. Electronic address: [email protected].
  • 2 Department of Medical Laboratory Science and Biotechnology, College of Medicine, China Medical University, Taichung 40402, Taiwan.
  • 3 Institute of New Drug Development, College of Medicine, China Medical University, Taichung 40402, Taiwan.
  • 4 Tsuzuki Institute for Traditional Medicine, Collage of Pharmacy, China Medical University, Taichung, 40402, Taiwan; Department of Pharmaceutical Sciences, Nihon Pharmaceutical University, Kitaadachi-gun, Saitama 362-0806, Japan.
  • 5 Department of Applied Cosmetology and Master Program of Cosmetic Sciences, Hungkuang University, Taichung 43302, Taiwan.
  • 6 Department of Medical Laboratory Science and Biotechnology, College of Medicine, China Medical University, Taichung 40402, Taiwan. Electronic address: [email protected].
PMID: 33031879 DOI: 10.1016/j.jconrel.2020.10.007
Abstract

Dictamnine is an active pharmaceutical ingredient in Dictamnus dasycarpus, a Chinese herbal medicine widely used for the treatment of skin inflammations such as atopic dermatitis (AD). Oxazolone has been demonstrated to induce significant skin inflammation and produce inflammatory cytokine expression identical to that of AD. An in vitro HaCaT inflammation model treated with dictamnine, which efficiently scavenged the Reactive Oxygen Species (ROS) and mitochondrial ROS (mROS), and it reduced interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) expression, NLRP3 inflammasome activation, and NF-κB expression. To explore the anti-inflammatory mechanism of dictamnine and enhance sustained drug release and penetration into epidermal structures in a dermatitis mouse model, we prepared PLGA-nanocarrier-encapsulated dictamnine (Dic-PLGA-NC) in a specifically designed bioreactor, namely an ultrasound composite streams-impinging mixer (U-SiM). Mouse dermatitis model was treated with Dic-PLGA-NC medication, spleens were collected to evaluate body weight ratio, and skin was retrieved for histological examination and two-photon microscopy. The data demonstrate that Dic-PLGA-NC efficiently penetrated the dermal layer, making it superior to naked dictamnine; moreover, it ameliorated the dermatitis symptoms and inflammatory cytokine expression in vivo. Dic-PLGA-NC produced using the U-SiM bioreactor could be used in new manufacturing processes for drugs to treat AD.

Keywords

Atopic dermatitis; Dictamnine; Drug delivery; Drug nanoformulation; Oxazolone; Polymeric nanocarrier.

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