2. Academic Validation
  3. Effects of valerate on intestinal barrier function in cultured Caco-2 epithelial cell monolayers

Effects of valerate on intestinal barrier function in cultured Caco-2 epithelial cell monolayers

  • Mol Biol Rep. 2022 Mar;49(3):1817-1825. doi: 10.1007/s11033-021-06991-w.
Guanzhen Gao 1,  Jingru Zhou 1,  Huiqin Wang 1,  Yanan Ding 1,  Jianwu Zhou 1,  Pik Han Chong 1,  Liying Zhu 2,  Lijing Ke 3,  Xin Wang 4,  Pingfan Rao 1,  Qiang Wang 5,  Longxin Zhang 6
Affiliations

Affiliations

  • 1 Food Nutrition Science Centre, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, China.
  • 2 State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China. [email protected].
  • 3 Food Nutrition Science Centre, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, China. [email protected].
  • 4 State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
  • 5 Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
  • 6 Fujian Provincial Maternity and Children Hospital, Affiliated hospital of Fujian Medical University, Fuzhou, China.
PMID: 34837149 DOI: 10.1007/s11033-021-06991-w
Abstract

Background: Short-chain fatty acids (SCFAs) are a group of microbial metabolites of undigested dietary fiber, protein and unabsorbed Amino acids in the colon, well-known for their gut health promoting benefits. A relatively high intestinal level of valerate was found in the healthy human subjects. However, the intestinal protection effects and the underlying mechanism of valerate are waiting to be verified and elucidated.

Methods and results: In the present study, valerate, a SCFAs mainly converted from proteins or Amino acids, was demonstrated to promote intestinal barrier function at its physiological concentrations of 0-4 mM in the Caco-2 cell monolayer model of intestinal barrier using transepithelial electrical resistance (TEER) assay and paracellular permeability assay. Valerate achieved the maximum increase in the TEER at 2 mM and reduced the paracellular permeability. Its intestinal barrier function promoting activity is similar to that of butyrate, with a broader range of effective concentrations than the later. Through western blot analysis, this activity is linked to the valerate-induced AMPK activation and tight junctions (TJs) assembly, but not to the reinforced expression of TJs related proteins.

Conclusions: It provides direct experimental evidence supporting valerate's function in intestinal health, implying the once under-valued function of valerate and its amino acid precursors. The valerate's role in regulating intestine homeostasis and its possible synergetic effects with other SCFAs warranted to be further investigated.

Keywords

AMPK activation; Caco-2 cell monolayer; Intestinal barrier function; Tight junction assembly; Valerate.

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