2. Academic Validation
  3. 3-Indoleacrylic acid from canola straw as a promising antialgal agent - Inhibition effect and mechanism on bloom-forming Prorocentrum donghaiense

3-Indoleacrylic acid from canola straw as a promising antialgal agent - Inhibition effect and mechanism on bloom-forming Prorocentrum donghaiense

  • Mar Pollut Bull. 2022 May:178:113657. doi: 10.1016/j.marpolbul.2022.113657.
Kokoette Effiong 1,  Jing Hu 1,  Caicai Xu 1,  Yiyi Zhang 1,  Shumiao Yu 1,  Tao Tang 1,  Yuzhou Huang 1,  Yongliang Lu 2,  Wei Li 3,  Jiangning Zeng 4,  Xi Xiao 5
Affiliations

Affiliations

  • 1 Department of Marine Science, Ocean College, Zhejiang University, Zhoushan 316021, People's Republic of China.
  • 2 China National Rice Research Institute, Hangzhou 310012, People's Republic of China.
  • 3 Academy of Agriculture and Forestry, Qinghai University, Xining 810016, People's Republic of China.
  • 4 Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, People's Republic of China.
  • 5 Department of Marine Science, Ocean College, Zhejiang University, Zhoushan 316021, People's Republic of China. Electronic address: [email protected].
PMID: 35452911 DOI: 10.1016/j.marpolbul.2022.113657
Abstract

Harmful algal blooms (HABs) have induced severe damage worldwide. A novel high-efficient antialgal natural chemical, 3-indoleacrylic acid (3-IDC) with a 5-day half-maximal inhibitory concentration (IC50, 5d), was discovered from canola straw, and its algal inhibition mechanism was investigated. Adverse effects were observed on the growth of P. donghaiense with 3-IDC addition, following an increase in Reactive Oxygen Species (ROS) production. 3-IDC also hindered the photosynthetic mechanism of P. donghaiense cells. Transcriptional results showed 3-IDC inhibiting the functions of all the nutrient assimilating genes, down-regulated ribulose-1,5-bisphosphate carboxylase/oxygenase II, and cytochrome f genes. The expression of heat shock protein (HSP) 70 and 90 and rhodopsin genes were also suppressed. The binding affinity of investigated receptors was observed. The conformational changes induced by the spatial microstructural alteration through 3-IDC may further contribute to the perturbation of those Enzyme catalytic activities. The present results provide new insights on controlling HABs using 3-IDC.

Keywords

3-indoleacrylic acid; Allelochemical; Harmful algae blooms; Molecular docking; Transcription.

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