1. Academic Validation
  2. PRMT5 Facilitates Infectious Bursal Disease Virus Replication through Arginine Methylation of VP1

PRMT5 Facilitates Infectious Bursal Disease Virus Replication through Arginine Methylation of VP1

  • J Virol. 2023 Feb 14;e0163722. doi: 10.1128/jvi.01637-22.
Xifeng Hu 1 2 Zheng Chen 1 2 Xiangdong Wu 1 2 Qiuling Fu 3 Zhen Chen 3 Yu Huang 3 Huansheng Wu 1 2
Affiliations

Affiliations

  • 1 Department of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, People's Republic of China.
  • 2 Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, People's Republic of China.
  • 3 Institute of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences, Fuzhou, People's Republic of China.
Abstract

The infectious bursal diseases virus (IBDV) polymerase, VP1 protein, is responsible for transcription, initial translation and viral genomic replication. Knowledge about the new kind of post-translational modification of VP1 supports identification of novel drugs against the virus. Because the arginine residue is known to be methylated by protein arginine methyltransferase (PRMT) Enzyme, we investigated whether IBDV VP1 is a substrate for known PRMTs. In this study, we show that VP1 is specifically associated with and methylated by PRMT5 at the arginine 426 (R426) residue. IBDV Infection causes the accumulation of PRMT5 in the cytoplasm, which colocalizes with VP1 as a punctate structure. In addition, ectopic expression of PRMT5 significantly enhances the viral replication. In the presence of PMRT5, Enzyme inhibitor and knockout of PRMT5 remarkably decreased viral replication. The polymerase activity of VP1 was severely damaged when R426 mutated to alanine, resulting in impaired viral replication. Our study reports a novel form of post-translational modification of VP1, which supports its polymerase function to facilitate the viral replication. IMPORTANCE Post-translational modification of infectious bursal disease virus (IBDV) VP1 is important for the regulation of its polymerase activity. Investigation of the significance of specific modification of VP1 can lead to better understanding of viral replication and can probably also help in identifying novel targets for Antiviral compounds. Our work demonstrates the molecular mechanism of VP1 methylation mediated by PRMT5, which is critical for viral polymerase activity, as well as viral replication. Our study expands a novel insight into the function of arginine methylation of VP1, which might be useful for limiting the replication of IBDV.

Keywords

IBDV VP1; PRMT5; R426; arginine methylation; polymerase activity; post-translation modification; veterinary microbiology; virology.

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