2. Academic Validation
  3. Molecular identification and functional analysis of X-linked inhibitor of apoptosis -associated factor-1 (XAF1) in grass carp, Ctenopharyngodon idella

Molecular identification and functional analysis of X-linked inhibitor of apoptosis -associated factor-1 (XAF1) in grass carp, Ctenopharyngodon idella

  • Fish Shellfish Immunol. 2023 Feb 22;134:108635. doi: 10.1016/j.fsi.2023.108635.
Jianping Fu 1,  Jun Chen 1,  XinYan Meng 1,  Zhang Luo 2,  Yi Liu 3,  Lili Wei 4
Affiliations

Affiliations

  • 1 College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi Province, 330022, PR China.
  • 2 Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, PR China.
  • 3 College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi Province, 330022, PR China. Electronic address: [email protected].
  • 4 College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi Province, 330045, PR China. Electronic address: [email protected].
PMID: 36822382 DOI: 10.1016/j.fsi.2023.108635
Abstract

X-linked inhibitor of Apoptosis protein (XIAP) -associated factor 1 (XAF1) is an interferon-stimulated gene which exhibits pro-apoptosis effect. In this study, XAF1 was characterized from grass carp Ctenopharyngodon idella and its expression pattern and function were analyzed. The open reading frame (orf) of XAF1 is 789 nucleotides (nt) encoding 262 Amino acids. SMART online search results showed that a C2H2-type and six C2HC-type zinc-fingers were found in XAF1, however, the XAF1 of grass carp showed high sequence identity to zebrafish (71%), low sequence identity to tetrapods (21-22%). Rt-qPCR results showed that XAF1 was constitutively expressed in all tested organs/tissues with highest expression in blood. An inductive expression of XAF1 at mRNA level was observed in peripheral blood leucocytes (PBLs) and C. idellus kidney cells (CIKs) after treatment with C. idellus recombinant interferon-γ (rIFNg). Overexpressing XAF1 in CIKs exhibited resistance against grass carp reovirus (GCRV) and more sensitivity to cisplatin. These results implied a functional homologue of XAF1 in evolution, however the mechanism may require further investigation.

Keywords

Apoptosis; Ctenopharyngodon idella; XAF1.

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