2. Academic Validation
  3. Near-infrared fluorophore IR-61 improves the quality of oocytes in aged mice via mitochondrial protection

Near-infrared fluorophore IR-61 improves the quality of oocytes in aged mice via mitochondrial protection

  • Biomed Pharmacother. 2023 Mar 27;162:114571. doi: 10.1016/j.biopha.2023.114571.
Jiadan Qu 1,  Lifeng Qin 2,  Jing Guo 3,  Ling Zhu 1,  Yunyao Luo 1,  Chong Li 1,  Juan Xie 1,  Jiaqiang Wang 2,  Chunmeng Shi 4,  Guoning Huang 5,  Jingyu Li 6
Affiliations

Affiliations

  • 1 Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Women and Children's Hospital of Chongqing Medical University, Chongqing, China; Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China.
  • 2 College of Life Science, Northeast Agricultural University, Harbin, China.
  • 3 College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
  • 4 Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing, China. Electronic address: [email protected].
  • 5 Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Women and Children's Hospital of Chongqing Medical University, Chongqing, China; Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China. Electronic address: [email protected].
  • 6 Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine, Women and Children's Hospital of Chongqing Medical University, Chongqing, China; Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China. Electronic address: [email protected].
PMID: 36989715 DOI: 10.1016/j.biopha.2023.114571
Abstract

Maternal aging is associated with a decline in oocyte quality, which leads to the decreased fertility. Therefore, developing approaches to reduce aging-induced deterioration of oocyte quality in older women is important. Near-infrared cell protector-61 (IR-61), a novel heptamethine cyanine dye, has the potential for antioxidant effects. In this study, we found that IR-61 can accumulate in the ovaries and improved ovarian function of naturally aged mice; it also increased the oocyte maturation rate and quality by maintaining the integrity of the spindle/chromosomal structure and reducing the aneuploidy rate. In addition, the embryonic developmental competence of aged oocytes was improved. Finally, RNA-sequencing analysis indicated that IR-61 might perform the beneficial effects on aged oocytes by regulating mitochondrial function, this was confirmed by immunofluorescence analysis of mitochondrial distribution and Reactive Oxygen Species. Taken together, our findings demonstrate that IR-61 supplementation in vivo can increase oocyte quality and protect oocytes from aging-induced mitochondrial dysfunction, and thus could improve the fertility of older women and efficiency of assisted reproductive technology.

Keywords

IR-61; Maternal aging; Mice; Mitochondrial function; Oocyte quality.

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