2. Academic Validation
  3. Regeneration of taste through sonic hedgehog upregulation by photobiomodulation

Regeneration of taste through sonic hedgehog upregulation by photobiomodulation

  • J Biophotonics. 2023 Jul 22;e202300043. doi: 10.1002/jbio.202300043.
Ha Young Lee 1,  Celine Dg Abueva 2,  Andrew Padalhin 2,  So Young Park 2,  Hyun Seok Ryu 1,  Phil-Sang Chung 2 3,  Hee Jung Kim 4,  Jeongyun Kim 5,  Seung Hoon Woo 2 3
Affiliations

Affiliations

  • 1 Interdisciplinary Program for Medical Laser, Dankook University, Cheonan, Republic of Korea.
  • 2 Dankook Institute of Medicine and Optics, Dankook University, Cheonan, Republic of Korea.
  • 3 Department of Otorhinolaryngology-Head and Neck Surgery, Dankook University Hospital, Cheonan, Republic of Korea.
  • 4 Department of Physiology, College of Medicine, Dankook University, Cheonan, Republic of Korea.
  • 5 Department of Physics, Dankook University, Cheonan, Republic of Korea.
PMID: 37483112 DOI: 10.1002/jbio.202300043
Abstract

This study investigated photobiomodulation (PBM) effects in Sonic Hedgehog (Shh) signaling as a potential approach to taste preservation and regeneration. Primary taste cell (TC) cultures were treated with Shh antagonist vismodegib and irradiated using a continuous wave type 630 nm light-emitting diode (10 mW/cm2 ) array, with single or multiple doses of 30 J/cm2 to determine dose inducing significant upregulation effect. Shh, Ptch, Smo, and Gli1 were significantly upregulated at 120 J/cm2 , used as the minimum dose in vivo. Vismodegib was administered via daily oral gavage for 21 days (30 mg/kg) to induce Shh inhibition in the tongue of rat animal models resulting in taste bud damage and taste dysfunction. PBM treatment using a 630 nm laser (3 W/cm2 ) at a radiant exposure of 120 J/cm2 (24 J/cm2 × 5) successfully upregulated the Shh protein expression, regenerated taste buds, and recovered taste function.

Keywords

photobiomodulation; sonic hedgehog; taste; taste regeneration.

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