2. Academic Validation
  3. Transcriptome Analysis of Norepinephrine-induced Lipolysis in Differentiated Adipocytes of Bama Pig

Transcriptome Analysis of Norepinephrine-induced Lipolysis in Differentiated Adipocytes of Bama Pig

  • Gene. 2023 Aug 31;147753. doi: 10.1016/j.gene.2023.147753.
Li He 1,  Hong Li 1,  Linzhen Zhang 1,  Jiaman Zhang 1,  Gen Zhang 1,  Xingyan Tong 1,  Tingting Zhang 1,  Yifan Wu 1,  Mingzhou Li 2,  Long Jin 3
Affiliations

Affiliations

  • 1 Sichuan Provincial Key Laboratory of Animal Breeding and Genetics, Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China.
  • 2 Sichuan Provincial Key Laboratory of Animal Breeding and Genetics, Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: [email protected].
  • 3 Sichuan Provincial Key Laboratory of Animal Breeding and Genetics, Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: [email protected].
PMID: 37659599 DOI: 10.1016/j.gene.2023.147753
Abstract

Sympathetic innervation of white adipose tissue (WAT) plays a key role in the regulation of lipid metabolism. Sympathetic activation promotes release of norepinephrine (NE), which binds to adrenergic receptors on adipocytes, promoting adipocyte lipolysis and enhanced oxidative metabolism. However, the mechanism by which sympathetic nerves regulate lipid metabolism in pig adipose tissue remains unclear. We used NE to simulate the process of sympathetic driving in pig adipocytes. RNA sequencing (RNA-seq) was used to determine the gene expression profile of pig adipocytes responding to NE stimulation. Our data suggests that the lipolytic signaling pathway is activated in pig adipocytes upon acute stimulation of NE, resulting in enhanced lipid metabolism and lipolysis, consistent with the phenomena found in humans and mice. Specifically, differentially expressed protein coding genes (PCGs) (SIRT4, SLC27A1) are mainly associated with functions that inhibit fatty acid oxidation and promote lipid synthesis. Similarly, we investigated the changes in regulatory transcripts such as long non-coding RNAs (lncRNAs) and transcripts of uncertain coding potential (TUCP) in response to NE and found that differentially expressed lncRNAs (lncG47338, lncG30660, lncG29516, lncG3790) and TUCP (TUCP_G38001) were co-expressed with target genes related to the promotion of fatty acid β-oxidation, lipolysis and oxidative metabolism, thus acting as regulators. These results indicate a broad suite of gene expression alterations in response to NE stimulation and promote the understanding of the molecular mechanisms by which NE regulates lipid metabolism in pigs.

Keywords

Lipid metabolism; Norepinephrine; Pig; RNA-seq; Sympathetic nerves.

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