2. Academic Validation
  3. Echinococcus granulosus cyst fluid inhibits the type I interferon response by promoting ROS in macrophages

Echinococcus granulosus cyst fluid inhibits the type I interferon response by promoting ROS in macrophages

  • Acta Trop. 2023 Dec 13:107101. doi: 10.1016/j.actatropica.2023.107101.
Chunxue Fu 1,  Chun Yang 1,  Caiya Ni 1,  Lianghai Wang 2,  Jun Hou 3
Affiliations

Affiliations

  • 1 NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, the First Affiliated Hospital/Shihezi University School of Medicine, Shihezi, Xinjiang, China; Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
  • 2 NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, the First Affiliated Hospital/Shihezi University School of Medicine, Shihezi, Xinjiang, China; Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China. Electronic address: [email protected].
  • 3 NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, the First Affiliated Hospital/Shihezi University School of Medicine, Shihezi, Xinjiang, China; Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China. Electronic address: [email protected].
PMID: 38101763 DOI: 10.1016/j.actatropica.2023.107101
Abstract

In cystic echinococcosis (CE), Echinococcus granulosus cystic fluid (EgCF) could impede macrophage-mediated immunity. However, whether EgCF is implicated in the type I interferon response remains to be established. Here, we revealed that EgCF reduced 2'3'-cGAMP-induced IFN-β production in macrophages by inhibiting the cGAS-STING-IRF3 signaling. EgCF also increased the intracellular Reactive Oxygen Species (ROS) levels. Administration of the ROS inhibitor N-acetylcysteine (NAC) restored the cGAS-STING-IRF3 signaling, which, in turn, upregulated IFN-β expression. The findings disclose that EgCF could increase macrophage ROS levels, thereby blocking cGAS-STING-IRF3 signaling and repressing the IFN-I response.

Keywords

Echinococcus granulosus; Macrophages; ROS; Type I Interferon; cGAS-STING.

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