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  3. Study transport of hesperidin based on the DPPC lipid model and the BSA transport model

Study transport of hesperidin based on the DPPC lipid model and the BSA transport model

  • Spectrochim Acta A Mol Biomol Spectrosc. 2024 Mar 18:314:124172. doi: 10.1016/j.saa.2024.124172.
Hong Zhuang 1,  Xiaoliang Zhang 1,  Sijia Wu 1,  Chen Mao 1,  Yaxi Dai 1,  Pang Yong 1,  Xiaodi Niu 2
Affiliations

Affiliations

  • 1 College of Food Science and Engineering, Jilin University, Changchun, Jilin 130062, China.
  • 2 College of Food Science and Engineering, Jilin University, Changchun, Jilin 130062, China. Electronic address: [email protected].
PMID: 38513316 DOI: 10.1016/j.saa.2024.124172
Abstract

Hesperidin (HE), a significant flavonoid polyphenolic compound present in citrus Plants, exhibits diverse pharmacological effects. Considering the crucial involvement of biological membranes and transporter proteins in the transportation and biological processes of HE, it becomes essential to comprehend the potential mechanisms through which HE interacts with membranes and transporter proteins. In order to simulate the process of active molecule transport, a cell membrane model consisting of 1,2-dipalmitoyl-n-glycero-3-phosphatidylcholine (DPPC) and a transporter protein model of bovine serum albumin (BSA) were employed for investigation. The present study aimed to investigate the mechanism of action of hesperidin (HE) in DPPC and BSA using fluorescence quenching, Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). The localization and interaction of HE within liposomes were also elucidated. Furthermore, the binding of BSA and HE was analyzed through UV/Vis absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and computational biology techniques. Computational biology analysis revealed that the binding between HE and BSA primarily occurred via hydrogen bonding and hydrophobic interactions. This study aimed to investigate the role and mechanism of HE in the DPPC cell membrane model and the BSA transporter protein model, thereby offering novel insights into the action of HE in DPPC and BSA.

Keywords

Biological membranes; Hesperidin; Transporter protein.

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