Mitochondrial fusion promoter M1

Name: Mitochondrial fusion promoter M1
Brand: MedChemExpress
SKU: HY-111475
Rating: 4.5 (3 reviews)

Cat. No.: HY-111475 Purity: 99.95%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Mitochondrial fusion promoter M1 is a mitochondrial dynamic modulator. Mitochondrial fusion promoter M1 preserves the mitochondrial function and promotes cellular respiration. Mitochondrial fusion promoter M1 alleviates cardiac and brain damage in rats with cardiac ischemia/reperfusion injury.

For research use only. We do not sell to patients.

Mitochondrial fusion promoter M1 Chemical Structure

CAS No. : 219315-22-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 99	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 275	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

1 Publications Citing Use of MCE Mitochondrial fusion promoter M1

•Ecotoxicol Environ Saf. 2023 Apr 5;256:114876. [Abstract]

: Mitochondrial fusion promoter M1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Apr 5;256:114876. [Abstract]; Mitochondrial fusion promoter M1 (M1; 1 μM;12 h) significantly attenuates TPHP-induced mitochondrial reduction and abnormal alignment in TM3 cells.

: Mitochondrial fusion promoter M1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Apr 5;256:114876. [Abstract]; Mitochondrial fusion promoter M1 (M1; 1 μM;12 h) significantly increases the TPHP-induced expression of mitochondrial fusion proteins Mfn1, Mfn2 and Opa1 in TM3 cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Mitochondrial fusion promoter M1 (5-25 μM; 24 h) promotes mitochondrial elongation in both Mitofusin-1 and Mitofusin -2 knock-out fibroblasts^[2].
Mitochondrial fusion promoter M1 (20 μM; 12 h) decreases mito ROS to 1.0±0.44 fold, enhances mitochondrial membrane potential from 0.29±0.05 fold to 0.5±0.07 fold and restores mitochondrial architecture in BRIN-BD11 pancreatic beta cells^[3].
Mitochondrial fusion promoter M1 (20 μM; 12 h) prevents the impairment of oxygen consumption rate in pancreatic beta cells due to cholesterol exposure^[3].
Mitochondrial fusion promoter M1 (20 μM; 12 h) prevents the impairment of non-mitochondrial respiration and extracellular acidification rate (ECAR) in cholesterol enriched pancreatic beta cells^[3].
Mitochondrial fusion promoter M1 (20 μM; 12 h) restores Glucose Stimulated Insulin Secretion (GSIS) in cholesterol treated pancreatic beta cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Mitochondrial fusion promoter M1 (2 mg/kg; i.v.) significantly protects rats with cardiac I/R injury against brain damage^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats (250-300g) receiving cardiac ischemia/reperfusion (I/R)^[1]
Dosage:	2 mg/kg
Administration:	I.v. 15 minutes before cardiac I/R injury
Result:	Increased brain mitochondrial fusion. Increased blood-brain barrier (BBB) tight junction protein, and reduced macrophage inﬁltration in the brain. Reduced brain mitochondrial dysfunction and apoptosis, but it did not reduce mitochondrial oxidative stress. Reduced the expression of Alzheimer’s disease (AD)-related proteins.

Molecular Weight

364.05

Formula

C₁₄H₁₀Cl₄N₂O

CAS No.

219315-22-7

Appearance

Solid

Color

White to off-white

SMILES

C/C(C1=CC(Cl)=CC=C1O)=N\NC2=C(Cl)C=C(Cl)C=C2Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 41.67 mg/mL (114.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7469 mL	13.7344 mL	27.4688 mL
5 mM	0.5494 mL	2.7469 mL	5.4938 mL
10 mM	0.2747 mL	1.3734 mL	2.7469 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 0.5 mg/mL (1.37 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 0.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.5 mg/mL (1.37 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (247 KB) HNMR (260 KB) RP-HPLC (244 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Surinkaew P, et, al. Mitochondrial Fusion Promoter Alleviates Brain Damage in Rats with Cardiac Ischemia/Reperfusion Injury. J Alzheimers Dis. 2020;77(3):993-1003. [Content Brief]

[2]. Wang D, et, al. A small molecule promotes mitochondrial fusion in mammalian cells. Angew Chem Int Ed Engl. 2012 Sep 10;51(37):9302-5. [Content Brief]

[3]. Asalla S, et, al. Restoring Mitochondrial Function: A Small Molecule-mediated Approach to Enhance Glucose Stimulated Insulin Secretion in Cholesterol Accumulated Pancreatic beta cells. Sci Rep. 2016 Jun 10;6:27513. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7469 mL	13.7344 mL	27.4688 mL	68.6719 mL
	5 mM	0.5494 mL	2.7469 mL	5.4938 mL	13.7344 mL
	10 mM	0.2747 mL	1.3734 mL	2.7469 mL	6.8672 mL
	15 mM	0.1831 mL	0.9156 mL	1.8313 mL	4.5781 mL
	20 mM	0.1373 mL	0.6867 mL	1.3734 mL	3.4336 mL
	25 mM	0.1099 mL	0.5494 mL	1.0988 mL	2.7469 mL
	30 mM	0.0916 mL	0.4578 mL	0.9156 mL	2.2891 mL
	40 mM	0.0687 mL	0.3434 mL	0.6867 mL	1.7168 mL
	50 mM	0.0549 mL	0.2747 mL	0.5494 mL	1.3734 mL
	60 mM	0.0458 mL	0.2289 mL	0.4578 mL	1.1445 mL
	80 mM	0.0343 mL	0.1717 mL	0.3434 mL	0.8584 mL
	100 mM	0.0275 mL	0.1373 mL	0.2747 mL	0.6867 mL