2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Neuronal Signaling
  3. Reactive Oxygen Species Cholinesterase (ChE)
  4. NLRP3-IN-33

NLRP3-IN-33 (Compound 12o) is a blood-brain barrier permeable inhibitor of AChE and BChE, with IC50 values of 1.02 μM and 7.03 μM against hAChE and hBChE respectively. NLRP3-IN-33 possesses antioxidant, anti-inflammatory, and metal chelating activities, making it a potential candidate for research in Alzheimer's disease (AD).

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NLRP3-IN-33 Chemical Structure

NLRP3-IN-33 Chemical Structure

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NLRP3-IN-33 Related Antibodies

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Description

NLRP3-IN-33 (Compound 12o) is a blood-brain barrier permeable inhibitor of AChE and BChE, with IC50 values of 1.02 μM and 7.03 μM against hAChE and hBChE respectively. NLRP3-IN-33 possesses antioxidant, anti-inflammatory, and metal chelating activities, making it a potential candidate for research in Alzheimer's disease (AD)[1].

IC50 & Target

IC50: 1.02 Μm (hAChE)[1].
IC50: 7.03 μM (hBChE)[1].
In Vitro

NLRP3-IN-33 (12o) (1-30 μM; 24 h) exhibits no significant cytotoxicity in PC-12 cells[1]. NLRP3-IN-33 possesses antioxidant activity and can inhibit the generation of free radicals, with an IC50 value of 6.19 μM. 12o (1-20 μM; 24 h) effectively alleviates H2O2 (600 μM; 24 h)-induced oxidative stress and exhibits neuroprotective effects in PC-1 cells[1]. NLRP3-IN-33 (1-20 μM; 24 h) also inhibits the activation of the NLRP3 inflammasome in PC-1 cells and mitigates the damage caused by mitochondrial-induced reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) triggered by LPS (1 μg/mL) and ATP (5 mM) in HMC-3 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NLRP3-IN-33 Related Antibodies

Western Blot Analysis[1]

Cell Line: HMC-3
Concentration: 12.5 μM, 25 μM
Incubation Time: 24 h
Result: Inhibited the NLRP3 inflammasome activation and caspase-1 release.
Significantly decreased the expression of NF-κB and NLRP3 proteins.
In Vivo

LRP3-IN-33 (12o) (0.05-0.02 mg/mL) can more effectively reduce mitochondrial and cellular oxidative stress in Drosophila AD models at a lower dosage (0.05 mg/mL)[1].
NLRP3-IN-33 (5 mg/kg; i.p.; once daily for 22 consecutive days) is capable of improving memory and cognitive impairments in AD mouse models induced by scopolamine (HY-N0296) (1.4 mg/kg; i.p.; once daily for 5 consecutive days)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Alzheimer's disease (AD) fractional model[1]
Dosage: 1 mg/kg, 5 mg/kg
Administration: Intraperitoneal injection (i.p.); Once daily for 22 days. Received scopolamine (HY-N0296) (1.4 mg/kg; i.p.; once daily for 5 days) on the last 5 days of the experiment (day 18 to day 22).
Result: Significantly shortened escape latency time as compared to the scopolamine-treated group.
Molecular Weight

393.39

Formula

C21H19N3O5
SMILES

O=C(NCC(NC1=C2C=CC=NC2=C(O)C=C1)=O)/C=C/C3=CC=C(O)C(OC)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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NLRP3-IN-33 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NLRP3-IN-33Reactive Oxygen SpeciesCholinesterase (ChE)Alzheimer’sdisease (AD)PC-12HMC-3antioxidantanti-inflammatorymetal chelating activitiesInhibitorinhibitorinhibit

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