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  4. Poloxamer 188

Poloxamer 188  (Synonyms: PEG-PPG-PEG, 8800 (Average Mn))

Cat. No.: HY-D1005A
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Poloxamer 188 is a nonionic linear copolymer with surfactant properties. Poloxamer 188 exhibits anti-thrombotic, anti-inflammatory, and cytoprotective activities in various tissue injury models. Poloxamer 188 can be used for drug delivery.

For research use only. We do not sell to patients.

Poloxamer 188 Chemical Structure

Poloxamer 188 Chemical Structure

CAS No. : 9003-11-6

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Poloxamer 188 Related Antibodies

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Description

Poloxamer 188 is a nonionic linear copolymer with surfactant properties. Poloxamer 188 exhibits anti-thrombotic, anti-inflammatory, and cytoprotective activities in various tissue injury models. Poloxamer 188 can be used for drug delivery[1][2][3][4].

In Vitro

Poloxamer 188 (10-1000 μM, 5 h) has a protective effect on cerebral microvascular endothelial cells (MBEC) in mice[2].
Docetaxel-loaded PLGA/poloxamer 188 nanoparticles leads to an increased level of drug uptake and cytotoxicity in the docetaxel-resistant MCF-7 TAX30 human breast cancer cells against Docetaxel-loaded PLGA nanoparticles[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Poloxamer 188 Related Antibodies

Cell Viability Assay [2]

Cell Line: MBEC
Concentration: 10, 100, 1000 μM
Incubation Time: 5 h
Result: Increased the cell number of hypoxic cells at high concentrations.
In Vivo

Poloxamer 188 (150 mg/kg intravenously, 240 minutes apart) significantly reduces ischemia-reperfuse-associated muscular edema and lipid peroxidation[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: TourniquetInduced Ischemia-Reperfusion Injury in Rats[3]
Dosage: 150 mg/kg
Administration: i.v
Result: Reduced the elevated TBARS but not to control levels and SOD activity was at control levels.
Clinical Trial
Molecular Weight

8800(Average)

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

CC.[H(OCH2CH2)x(OCH2CH)y(OCH2CH2)zOH].[CH3]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL

H2O : ≥ 100 mg/mL

*"≥" means soluble, but saturation unknown.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (Infinity mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (Infinity mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 25 mg/mL (Infinity mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References
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Poloxamer 188 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Poloxamer 1889003-11-6PEG-PPG-PEG, 8800 (Average Mn)Poloxamer188Poloxamer-188Biochemical Assay ReagentsSurfactantAnti-inflammatoryAntioxidantMBECTBARSInhibitorinhibitorinhibit

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