3D cell culture is a technology that cultivates cells in a three-dimensional structure to simulate the environment closer to the body. Compared with traditional 2D culture, 3D culture provides more realistic cell interactions, tissue structure and cell function. This culture method is widely used in biomedical research, drug screening, and tissue engineering. By creating three-dimensional structures that more closely resemble the environment within an organism, 3D cell culture helps more accurately simulate biological processes such as disease development, drug response, and tissue reconstruction.