Copper death assay is an experimental method used to evaluate cell death under stress of high concentrations of copper ions. High concentrations of copper ions can cause oxidative stress and cell damage, ultimately leading to cell death. Detection methods include fluorescent probe labeling, cell morphology observation, DNA fragmentation, etc. Copper death detection helps to study the stress response of cells to copper stress and reveals the mechanism of copper's action in organisms and its relationship with disease occurrence. This research is important for a deeper understanding of the role of metal ions in biological processes.