Ferroptosis assay is an experimental method used to assess cell death due to iron stress. In the presence of high concentrations of iron ions, excessive free radicals can be generated within cells, leading to oxidative stress and cell damage. Detection methods include fluorescent probe labeling, cell morphology observation, DNA fragmentation, etc. Ferroptosis detection is helpful in studying the relationship between cellular iron metabolism disorders and diseases, such as anemia, neurological diseases, etc. This research is of great significance for revealing the mechanism of iron action in organisms and potential therapeutic targets.