Frozen sectioning is a method used to prepare sections for the study of cells and tissues. First, the tissue sample is encapsulated in cryogen by freezing to form a frozen block. Then, use a freezing microtome or microtome to cut thin sections at very low temperatures. This technology retains the natural structure and biological activity of cells and tissues, and is suitable for studies such as immunohistochemistry, fluorescence microscopy, and in situ hybridization. Frozen sectioning technology is important for preserving the activity of biomolecules and providing high-resolution images.