Protein extraction is the process of separating proteins from biological samples, providing the basis for biological research, drug development and clinical diagnosis. The technology involves steps such as cell disruption, protein solubilization and separation. Selecting the appropriate extraction buffer and method is key to ensure protein integrity and activity. The extracted protein can be used for immunoblotting, mass spectrometry analysis, enzyme activity determination and other applications, providing an important experimental basis for in-depth understanding of life activities and disease mechanisms.