2. NF-κB Apoptosis
  3. NF-κB Apoptosis
  4. PTD-p65-P1 Peptide TFA

PTD-p65-P1 Peptide TFA is a potent, selective nuclear transcription factor NF-κB inhibitor and derives from the p65 subunit of NF-κB amino acid residues 271-282, which selectively inhibits NF-κB activation induced by various inflammatory stimulation, down-regulate NF-κB-mediated gene expression and up-regulate apoptosis.

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PTD-p65-P1 Peptide TFA Chemical Structure

PTD-p65-P1 Peptide TFA Chemical Structure

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5 mg USD 300 In-stock
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Based on 3 publication(s) in Google Scholar

Other Forms of PTD-p65-P1 Peptide TFA:

PTD-p65-P1 Peptide TFA Related Antibodies

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Description

PTD-p65-P1 Peptide TFA is a potent, selective nuclear transcription factor NF-κB inhibitor and derives from the p65 subunit of NF-κB amino acid residues 271-282, which selectively inhibits NF-κB activation induced by various inflammatory stimulation, down-regulate NF-κB-mediated gene expression and up-regulate apoptosis[1][2].

IC50 & Target

NF-kappaB[1]
In Vitro

PTD-p65-P1 Peptide TFA (10-150 μM; 0-60 min; KBM-5 cells) inhibits TNF-induced NF-κB activation in a dose-dependent manner and suppresses TNF-induced NF-κB activation by 25% at 100 μM and completely at 150 μM[1].
PTD-p65-P1 Peptide TFA (150 μM; 0-60 min; KBM-5 cells) inhibits cytoplasmic p65 phosphorylation and nuclear translocation[1].
PTD-p65-P1 Peptide TFA (100 μM; 16 h; KBM-5 cells) potentiates the TNF-induced apoptosis from 4 to 45%[1].
PTD-p65-P1 Peptide TFA (150 μM; A 293 cells) inhibits NF-κB-dependent reporter gene expression induced by TNF[1].
PTD-p65-P1 Peptide TFA (150 μM; 12-24 h; SP-53 cells ) inhibits the cell proliferation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PTD-p65-P1 Peptide TFA Related Antibodies

Western Blot Analysis[1]

Cell Line: KBM-5 cells
Concentration: 150 μM
Incubation Time: 0, 5, 10, 15, 30 and 60 minutes
Result: Suppressed TNF-induced NF-κB activation by inhibiting phosphorylation and nuclear translocation of p65.

Cell Proliferation Assay[2]

Cell Line: SP-53 cells
Concentration: 150 μM
Incubation Time: 12 and 24 hours
Result: Inhibited cell proliferation of 40% in 12 hours and 60% in 24 hours.
Molecular Weight

3943.52

Formula

C170H276F3N57O46S
Appearance

Solid

Color

White to off-white

Sequence Shortening

DRQIKIWFQNRRMKWKKQLRRPSDRELSE
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (25.36 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.2536 mL 1.2679 mL 2.5358 mL
5 mM 0.0507 mL 0.2536 mL 0.5072 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.14%

COA (241 KB) RP-HPLC (112 KB) MS (244 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.2536 mL 1.2679 mL 2.5358 mL 6.3395 mL
5 mM 0.0507 mL 0.2536 mL 0.5072 mL 1.2679 mL
10 mM 0.0254 mL 0.1268 mL 0.2536 mL 0.6340 mL
15 mM 0.0169 mL 0.0845 mL 0.1691 mL 0.4226 mL
20 mM 0.0127 mL 0.0634 mL 0.1268 mL 0.3170 mL
25 mM 0.0101 mL 0.0507 mL 0.1014 mL 0.2536 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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PTD-p65-P1 Peptide TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PTD-p65-P1 PeptideNF-κBApoptosisNuclear factor-κBNuclear factor-kappaBapoptosisTNFinflammatory stimulationKBM-5 cellsanticancerInhibitorinhibitorinhibit

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