2. Anti-infection Immunology/Inflammation Apoptosis Metabolic Enzyme/Protease
  3. HBV Pyroptosis Carbonic Anhydrase
  4. Punicalin

Punicalin is a species that can be isolated from the leaves of Punica granatum. Punicalin is an active molecule against hepatitis b virus (HBV). Punicalin can induce pyroptosis. Punicalin is a Carbonic anhydrase inhibitor. Punicalin blocks the binding of S-glycoprotein and ACE2 receptors. Pnuicalin has anti-inflammatory, antioxidant and antiviral activity.

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Punicalin Chemical Structure

Punicalin Chemical Structure

CAS No. : 65995-64-4

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Based on 1 publication(s) in Google Scholar

Punicalin Related Antibodies

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Description

Punicalin is a species that can be isolated from the leaves of Punica granatum. Punicalin is an active molecule against hepatitis b virus (HBV). Punicalin can induce pyroptosis. Punicalin is a Carbonic anhydrase inhibitor. Punicalin blocks the binding of S-glycoprotein and ACE2 receptors. Pnuicalin has anti-inflammatory, antioxidant and antiviral activity[1][2][3][4][5][6][7].

In Vitro

Punicalin (0-100 μM, 24 h) improves LPS/ ATP-induced pyroptosis in mouse mononuclear macrophage J774A by inhibiting ROS/NLRP3 pathway<[3].
Punicalin (10, 20 μM, 24 h) alleviates OGD/R-induced cell damage through TGF-β-mediated neuroblastoma SH-SY5Y oxidative stress and cell cycle[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Punicalin Related Antibodies

Western Blot Analysis[3]

Cell Line: J774A
Concentration: 0, 25, 50, 100 μM
Incubation Time: The cells were pretreated with punicalin for one hour, followed by treated with Lipopolysaccharide (HY-D1056) for 5.5 h, ATP for half an hour, respectively.
Result: Reduced the increased protein expression of NLRP3, ASC, Caspase-1 and GSDMD-N.

Cell Cycle Analysis[4]

Cell Line: SH-SY5Y
Concentration: 10, 20 μM
Incubation Time: 24-72 h
Result: Alleviated the rising of the G0/G1 phase ratio.
Ameliorated OGD/R-mediated expression of cyclin B1, CDK1, p21 and phosphorylation of CDK1 at 20 μM.

Cell Viability Assay[3]

Cell Line: J774A
Concentration: 0, 25, 50, 100 μM
Incubation Time: 24 h
Result: Promoted cell viability and decreased the release of pro-inflammatory cytokines.
In Vivo

Punicalin (5 mg/kg, subcutaneous injection) has an obvious anti-inflammatory effect on carrageenan (HY-125474)-induced metatarsal edema in rats[1].
Punicalin (10 mg/kg, intraperitoneal injection) alleviates LPS (HY-D1056)-induced acute lung injury in mice by inhibiting the production of inflammatory cytokines and MAPK/ NF-κB signaling[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Carrageenan (HY-125474)-induced Edema in rats[1]
Dosage: 5 mg/kg
Administration: s.c.
Result: Showed significant anti-inflammatory effect against carrageenan induced paw edema at all time intervals, but decreased the effects.with larger dose.
Animal Model: LPS (HY-D1056)-induced acute lung injury mice[5]
Dosage: 10 mg/kg
Administration: i.p.
Result: Reduced the mortality and attenuated the severity of lung pathological injury.
Ameliorated LPS (HY-D1056)-induced lung edema and vascular leakage.
Molecular Weight

782.53

Formula

C34H22O22
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C1C(C(C(C(O)=C(O)C(O)=C2)=C2C(OC(C3O)C(O)C(O)C=O)=O)=C(O)C(O)=C4OC5=O)=C4C6=C5C(C7=C(C(OC3)=O)C=C(O)C(O)=C7O)=C(O)C(O)=C6O1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (63.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (63.90 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2779 mL 6.3895 mL 12.7791 mL
5 mM 0.2556 mL 1.2779 mL 2.5558 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (63.90 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.89%

COA (262 KB) HNMR (344 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.2779 mL 6.3895 mL 12.7791 mL 31.9477 mL
5 mM 0.2556 mL 1.2779 mL 2.5558 mL 6.3895 mL
10 mM 0.1278 mL 0.6390 mL 1.2779 mL 3.1948 mL
15 mM 0.0852 mL 0.4260 mL 0.8519 mL 2.1298 mL
20 mM 0.0639 mL 0.3195 mL 0.6390 mL 1.5974 mL
25 mM 0.0511 mL 0.2556 mL 0.5112 mL 1.2779 mL
30 mM 0.0426 mL 0.2130 mL 0.4260 mL 1.0649 mL
40 mM 0.0319 mL 0.1597 mL 0.3195 mL 0.7987 mL
50 mM 0.0256 mL 0.1278 mL 0.2556 mL 0.6390 mL
60 mM 0.0213 mL 0.1065 mL 0.2130 mL 0.5325 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Punicalin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Punicalin65995-64-4HBVPyroptosisCarbonic AnhydraseHepatitis B virusCarbonate dehydrataseAnti-inflammatoryJ774ASH-SY5YLPS-inducedInhibitorinhibitorinhibit

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