2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Protease Inhibitors
  5. Serpin (Protease Inhibitor)
  6. Alpha 2 Antiplasmin
  7. Alpha-2-Antiplasmin/Serpin F2 Protein, Mouse (HEK293, His)

Alpha-2-Antiplasmin/Serpin F2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P71295
COA Handling Instructions

Alpha-2-Antiplasmin/Serpin F2 Protein, a serine protease inhibitor, targets plasmin and trypsin, also inhibiting matriptase-3/TMPRSS7 and chymotrypsin. It forms a heterodimer with TMPRSS7, enhancing its protease inhibiting activity. Alpha-2-Antiplasmin/Serpin F2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Alpha-2-Antiplasmin/Serpin F2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Alpha-2-Antiplasmin/Serpin F2 Protein, Mouse (HEK293, His) is 464 a.a., with molecular weight of 70-85 kDa.

For research use only. We do not sell to patients.

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Description

Alpha-2-Antiplasmin/Serpin F2 Protein, a serine protease inhibitor, targets plasmin and trypsin, also inhibiting matriptase-3/TMPRSS7 and chymotrypsin. It forms a heterodimer with TMPRSS7, enhancing its protease inhibiting activity. Alpha-2-Antiplasmin/Serpin F2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Alpha-2-Antiplasmin/Serpin F2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Alpha-2-Antiplasmin/Serpin F2 Protein, Mouse (HEK293, His) is 464 a.a., with molecular weight of 70-85 kDa.
Background

Alpha-2-Antiplasmin/Serpin F2 Protein is a serine protease inhibitor that primarily targets plasmin and trypsin, while also inhibiting matriptase-3/TMPRSS7 and chymotrypsin. Additionally, it forms a heterodimer with TMPRSS7 to further enhance its protease inhibiting activity.
Species

Mouse
Source

HEK293
Tag

C-6*His
Accession

Q61247 (V28-K491)
Gene ID

18816  [NCBI]
Molecular Construction
N-term
Alpha-2-AP (V28-K491)
Accession # Q61247
6*His
C-term
Synonyms
Alpha-2-antiplasmin; Alpha-2-AP; Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2; Serpinf2
AA Sequence

VDLPGQQPVSEQAQQKLPLPALFKLDNQDFGDHATLKRSPGHCKSVPTAEETRRLAQAMMAFTTDLFSLVAQTSTSSNLVLSPLSVALALSHLALGAQNQTLHSLHRVLHMNTGSCLPHLLSHFYQNLGPGTIRLAARIYLQKGFPIKDDFLEQSERLFGAKPVKLTGKQEEDLANINQWVKEATEGKIEDFLSELPDSTVLLLLNAIHFHGFWRTKFDPSLTQKDFFHLDERFTVSVDMMHAVSYPLRWFLLEQPEIQVAHFPFKNNMSFVVVMPTYFEWNVSEVLANLTWDTLYHPSLQERPTKVWLPKLHLQQQLDLVATLSQLGLQELFQGPDLRGISEQNLVVSSVQHQSTMELSEAGVEAAAATSVAMNRMSLSSFTVNRPFLFFIMEDTIGVPLFVGSVRNPNPSALPQLQEQRDSPDNRLIGQNDKADFHGGKTFGPDLKLAPRMEEDYPQFSSPK

Molecular Weight

70-85 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Solution.
Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 20% Glycerol, 1 mM DTT, pH 8.0.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation

COA (220 KB)

Handling Instructions (2659 KB)

Alpha-2-Antiplasmin/Serpin F2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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