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CARHSP1 Protein, Human

Cat. No.: HY-P76766A
COA Handling Instructions

The CARHSP1 protein critically regulates post-transcriptional gene expression by binding to mRNA, affecting its stability and influencing RNA homeostasis. Furthermore, in vitro binding of CARHSP1 to single-stranded DNA demonstrates the versatility of the molecular interaction. CARHSP1 Protein, Human is the recombinant human-derived CARHSP1 protein, expressed by E. coli , with tag free. The total length of CARHSP1 Protein, Human is 146 a.a., with molecular weight of 18-23 kDa.

For research use only. We do not sell to patients.

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10 μg $85 In-stock
50 μg $235 In-stock
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Description

The CARHSP1 protein critically regulates post-transcriptional gene expression by binding to mRNA, affecting its stability and influencing RNA homeostasis. Furthermore, in vitro binding of CARHSP1 to single-stranded DNA demonstrates the versatility of the molecular interaction. CARHSP1 Protein, Human is the recombinant human-derived CARHSP1 protein, expressed by E. coli , with tag free. The total length of CARHSP1 Protein, Human is 146 a.a., with molecular weight of 18-23 kDa.

Background

The CARHSP1 Protein plays a crucial role in post-transcriptional gene regulation by binding to mRNA and modulating the stability of the target mRNA, highlighting its involvement in cellular processes related to RNA homeostasis. Additionally, CARHSP1 exhibits the capacity to bind single-stranded DNA in vitro, suggesting potential versatility in its molecular interactions. Structurally, the protein forms homodimers, indicating that its functional activity may be linked to its dimeric state. Furthermore, CARHSP1 interacts with STYX, emphasizing its association with specific binding partners and potential involvement in larger molecular complexes or signaling pathways.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q9Y2V2 (S2-S147)

Gene ID

23589

Molecular Construction
N-term
CARHSP1 (S2-S147)
Accession # Q9Y2V2
C-term
Synonyms
Calcium-regulated heat-stable protein 1; Calcium-regulated heat-stable protein of 24 kDa; CRHSP-24
AA Sequence

SSEPPPPPQPPTHQASVGLLDTPRSRERSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS

Molecular Weight

18-23 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CARHSP1 Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CARHSP1 Protein, Human
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HY-P76766A
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