CD24 Protein, Mouse (27a.a, HEK293, Fc)

The CD24 protein may coordinate cellular differentiation of various cell types, particularly in early thymocyte development. Activation of its signaling involves binding of a lectin-like ligand to the CD24 carbohydrate, triggering the release of second messengers from GPI anchors. CD24 Protein, Mouse (27a.a, HEK293, Fc) is the recombinant mouse-derived CD24 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD24 Protein, Mouse (27a.a, HEK293, Fc) is 27 a.a., with molecular weight of 39-56 kDa.

CD24 Protein is postulated to play a pivotal role in the cell differentiation processes of various cell types, with a specific implication in early thymocyte development. Its signaling potential is suggested to be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, transduced through the release of second messengers derived from the GPI-anchor. Additionally, CD24 is implicated in modulating B-cell activation responses, indicative of its regulatory influence in immune processes. In conjunction with SIGLEC10, CD24 may participate in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. This CD24:SIGLEC10 complex is proposed to inhibit HMGB1-mediated tissue damage immune responses, suggesting CD24's role in immune regulation and control of autoimmunity. The multifaceted functions of CD24 underscore its potential as a key player in diverse cellular and immune processes.

Immobilized Mouse CD24 at 10 μg/mL (100 μL/well) can bind Anti-CD24 Antibody, The ED₅₀ for this effect is 0.1823 μg/mL, corresponding to a specific activity is 5.485×10^3 units/mg.

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  Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells. The ED₅₀ for this effect is 0.02271 ng/mL, corresponding to a specific activity is 4.40×10⁷ Unit/mg.

Mouse

HEK293

C-hFc

P24807 (N27-G53)

12484  [NCBI]

NQTSVAPFPGNQNISASPNPSNATTRG

39-56 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.