Enolase 1/ENO1 Protein, Mouse (His)

Enolase 1/ENO1 Proteins, as a glycolytic enzyme, convert 2-phosphoglycerate to phosphoenolpyruvate and have diverse roles in cellular processes like growth control, hypoxia tolerance, and allergic responses. They also function in the fibrinolytic system and stimulate immunoglobulin production, showing their involvement in immune responses. Enolase 1/ENO1 Protein, Mouse (His) is the recombinant mouse-derived Enolase 1/ENO1 protein, expressed by E. coli , with N-10*His labeled tag. The total length of Enolase 1/ENO1 Protein, Mouse (His) is 432 a.a., with molecular weight of ~50.4 kDa.

Enolase 1 (ENO1) is a glycolytic enzyme responsible for catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Beyond its role in glycolysis, ENO1 plays a multifaceted role in various cellular processes, including growth control, hypoxia tolerance, and allergic responses. Notably, it functions in the intravascular and pericellular fibrinolytic system by acting as both a receptor and activator of plasminogen on the cell surface, observed in diverse cell types such as leukocytes and neurons. Additionally, ENO1 exhibits the capacity to stimulate immunoglobulin production, further highlighting its involvement in immune responses.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

E. coli

N-10*His

P17182 (S2-A433)

13806  [NCBI]

SILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLYKSFVQNYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLA

Approximately 50.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm solution of Tris-based buffer, 50% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.