FGFR-1 Protein, Rhesus Macaque (HEK293, His)

FGFR-1 protein is a tyrosine-protein kinase that acts as a cell surface receptor for fibroblast growth factor and plays a critical role in the complex regulation of embryonic development, cell proliferation, differentiation, and migration. FGFR-1 mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1, MAP kinase signaling pathways, and AKT1 signaling pathways. FGFR-1 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived FGFR-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of FGFR-1 Protein, Rhesus Macaque (HEK293, His) is 262 a.a., with molecular weight of 50-75 kDa.

FGFR-1 protein is a tyrosine-protein kinase that acts as a cell surface receptor for fibroblast growth factor and plays a critical role in the complex regulation of embryonic development, cell proliferation, differentiation, and migration. FGFR-1 activates multiple signaling cascades, phosphorylating key proteins such as PLCG1, FRS2, GAB1, and SHB. This activation results in the production of signaling molecules via PLCG1. FRS2 phosphorylation triggers recruitment of GRB2, GAB1, PIK3R1, and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1, MAP kinase signaling pathways, and AKT1 signaling pathways. FGFR1 also promotes phosphorylation of SHC1, STAT1, and PTPN11/SHP2. In the nucleus, it can enhance the activity of RPS6KA1 and CREB1 and participate in transcriptional regulation. Down-regulation of FGFR1 signaling occurs through IL17RD/SEF and FGFR1 ubiquitination, internalization, and degradation^[1][2][3]

Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED₅₀ for this effect is typically 0.3610ng/mL, corresponding to a specific activity is 2.770×10⁶ units/mg.

Rhesus Macaque

HEK293

C-6*His

H9FRD4/NP_001253576.1 (R22-Y283)

700126  [NCBI]

RPSPTLPEQDALPSSEDDDDDDDSSSEEKETDNTKPNPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLY

Approximately 50-75 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.