FOLR3 Protein, Human (HEK293, His)

FOLR3 protein is crucial in folate metabolism, binding to folate and aiding 5-methyltetrahydrofolate delivery into cells. Notably, the Isoform Short of FOLR3 lacks folate-binding capabilities, indicating diverse functional roles among FOLR3 isoforms. This highlights nuanced regulation in cellular folate transport processes. FOLR3 Protein, Human (HEK293, His) is the recombinant human-derived FOLR3 protein, expressed by HEK293, with C-His labeled tag. The total length of FOLR3 Protein, Human (HEK293, His) is 223 a.a., with molecular weight of 33-45 kDa.

The FOLR3 protein plays a pivotal role in folate metabolism, binding to folate and reduced folic acid derivatives while facilitating the delivery of 5-methyltetrahydrofolate to the interior of cells. It is noteworthy that the Isoform Short of FOLR3 does not exhibit folate-binding capabilities. This differential binding behavior suggests distinct functional roles for the various isoforms of FOLR3, emphasizing the nuanced regulation of folate transport within cellular processes.

Human FOLR3 protein captured on CM5 chip can bind Folic acid with an affinity constant of 0.122 nM as detected by SPR assay(Biacore T200).

Human

HEK293

C-His

P41439 (Q23-S245)

2352  [NCBI]

33-45 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.