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  5. Glypican-5/GPC5 Protein, Human (sf9, His)

Glypican-5/GPC5 Protein, Human (sf9, His)

Cat. No.: HY-P73079
Handling Instructions

Glypican-5 (GPC5) protein is a cell surface proteoglycan characterized by specific binding to heparan sulfate. As a proteoglycan, GPC5 consists of a core protein covalently linked to heparan sulfate chains. Glypican-5/GPC5 Protein, Human (sf9, His) is the recombinant human-derived Glypican-5/GPC5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Glypican-5/GPC5 Protein, Human (sf9, His) is 530 a.a., with molecular weight of ~60.5 kDa.

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Description

Glypican-5 (GPC5) protein is a cell surface proteoglycan characterized by specific binding to heparan sulfate. As a proteoglycan, GPC5 consists of a core protein covalently linked to heparan sulfate chains. Glypican-5/GPC5 Protein, Human (sf9, His) is the recombinant human-derived Glypican-5/GPC5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Glypican-5/GPC5 Protein, Human (sf9, His) is 530 a.a., with molecular weight of ~60.5 kDa.
Background

Glypican-5 (GPC5) protein is a cell surface proteoglycan that is distinguished by its specific association with heparan sulfate. As a proteoglycan, GPC5 consists of a core protein that is covalently attached to heparan sulfate chains. The presence of this protein on the cell surface suggests its involvement in cellular signaling and communication. Heparan sulfate chains, which are known for their ability to interact with various growth factors and other signaling molecules, likely contribute to GPC5's role in modulating signaling pathways and cellular processes. The unique characteristics of GPC5, including its association with heparan sulfate, suggest its significant contribution to cellular functions and highlight its potential as a target for further research and therapeutic interventions.
Species

Human
Source

Sf9 insect cells
Tag

C-His
Accession

P78333 (E25-T554)
Gene ID
Molecular Construction
N-term
GPC5 (E25-T554)
Accession # P78333
His
C-term
Synonyms
Glypican proteoglycan 5; GPC5
AA Sequence

MDAQTWPVGFRCLLLLALVGSARSEGVQTCEEVRKLFQWRLLGAVRGLPDSPRAGPDLQVCISKKPTCCTRKMEERYQIAARQDMQQFLQTSSSTLKFLISRNAAAFQETLETLIKQAENYTSILFCSTYRNMALEAAASVQEFFTDVGLYLFGADVNPEEFVNRFFDSLFPLVYNHLINPGVTDSSLEYSECIRMARRDVSPFGNIPQRVMGQMGRSLLPSRTFLQALNLGIEVINTTDYLHFSKECSRALLKMQYCPHCQGLALTKPCMGYCLNVMRGCLAHMAELNPHWHAYIRSLEELSDAMHGTYDIGHVLLNFHLLVNDAVLQAHLNGQKLLEQVNRICGRPVRTPTQSPRCSFDQSKEKHGMKTTTRNSEETLANRRKEFINSLRLYRSFYGGLADQLCANELAAADGLPCWNGEDIVKSYTQRVVGNGIKAQSGNPEVKVKGIDPVINQIIDKLKHVVQLLQGRSPKPDKWELLQLGSGGGMVEQVSGDCDDEDGCGGSGSGEVKRTLKITDWMPDDMNFSDVKQIHQTDTGSTLDTTGAGCAVAT

Molecular Weight

Approximately 60.5 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Glypican-5/GPC5 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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