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  4. SP-17 Protein, Mouse (His)

SP-17 Protein, Mouse (His)

Cat. No.: HY-P701070
Handling Instructions

SP-17 protein acts as a sperm surface zona pellucida binding protein, facilitating high-affinity spermatozoa binding to the zona pellucida. It potentially engages in binding interactions with zona pellucida and carbohydrates. Existing as a homodimer, SP-17 may interact with ROPN1, suggesting a cooperative role in sperm-egg interactions and fertilization processes. SP-17 Protein, Mouse (His) is the recombinant mouse-derived SP-17 protein, expressed by E. coli , with N-His labeled tag. The total length of SP-17 Protein, Mouse (His) is 149 a.a., with molecular weight of 20-25 kDa.

For research use only. We do not sell to patients.

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Description

SP-17 protein acts as a sperm surface zona pellucida binding protein, facilitating high-affinity spermatozoa binding to the zona pellucida. It potentially engages in binding interactions with zona pellucida and carbohydrates. Existing as a homodimer, SP-17 may interact with ROPN1, suggesting a cooperative role in sperm-egg interactions and fertilization processes. SP-17 Protein, Mouse (His) is the recombinant mouse-derived SP-17 protein, expressed by E. coli , with N-His labeled tag. The total length of SP-17 Protein, Mouse (His) is 149 a.a., with molecular weight of 20-25 kDa.
Background

The SP-17 protein serves as a sperm surface zona pellucida binding protein, facilitating the high-affinity binding of spermatozoa to the zona pellucida. Its potential role includes participation in the binding interactions with zona pellucida and carbohydrates. Existing as a homodimer, SP-17 may also engage in interactions with ROPN1, indicating a possible cooperative role in the processes related to sperm-egg interactions and fertilization.
Species

Mouse
Source

E. coli
Tag

N-His
Accession

Q62252 (M1-N149)
Gene ID

20686  [NCBI]
Molecular Construction
N-term
His
SP-17 (M1-N149)
Accession # Q62252
C-term
Synonyms
Sperm surface protein Sp17; CT22; Sp17-1; Sperm protein 17; SPA17;
Molecular Weight

20-25 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

SP-17 Protein, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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