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  5. SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His)

SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His)

Cat. No.: HY-P74534
Handling Instructions

SUC2 gene is a unlinked loci of the S. cerevisiae genome. SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His) is the recombinant SUC2 protein, expressed by P. pastoris , with C-His labeled tag. The total length of SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His) is 513 a.a., with molecular weight of ~60 kDa.

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Description

SUC2 gene is a unlinked loci of the S. cerevisiae genome[1]. SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His) is the recombinant SUC2 protein, expressed by P. pastoris , with C-His labeled tag. The total length of SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His) is 513 a.a., with molecular weight of ~60 kDa.
Background

SUC2 gene is a unlinked loci of the S. cerevisiae genome[1]. SUC2gene encodes two forms of invertase: one is secreted glycosylated form, the synthesis of which is regulated by glucose inhibition, and the other is a constitutively produced intracellular nonglycosylated enzyme[2]. SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His) enables beta-fructofuranosidase activity and inulinase activity and is involved in the catabolism of inulin, raffinose and sucrose[3][4][5].
Species

Others
Source

P. pastoris
Tag

C-His
Accession

P00724 (S20-K532)
Gene ID

854644  [NCBI]
Molecular Construction
N-term
SUC2 (S20-K532)
Accession # P00724
His
C-term
Synonyms
Invertase 2; Saccharase; SUC2; Beta-fructofuranosidase 2
Molecular Weight

Approximately 60 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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