TCN2/Transcobalamin-2 Protein, Mouse (HEK293, C-His)

The TCN2/Transcobalamin-2 protein plays a key role as the primary vitamin B12 binding and transporter, ensuring efficient delivery of cobalamin to cells. Its complex interactions involve binding to CD320, specifically to the LDL receptor class A domain. TCN2/Transcobalamin-2 Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived TCN2/Transcobalamin-2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TCN2/Transcobalamin-2 Protein, Mouse (HEK293, C-His) is 412 a.a., with molecular weight of ~42.4 kDa.

The TCN2/Transcobalamin-2 protein takes on the pivotal role of being the primary vitamin B12-binding and transport protein, facilitating the delivery of cobalamin to cells. Through its intricate interactions, TCN2 engages with CD320, specifically binding to LDL-receptor class A domains. This molecular partnership suggests a coordinated mechanism for the targeted delivery of vitamin B12 to cells, emphasizing the protein's crucial role in the efficient transport and uptake of this essential nutrient.

Measured by its binding ability in a functional ELISA. Immobilized CD320 at 25 ng/mL (100 μL/well) can bind Biotinylated TCN2. The ED₅₀ for this effect is 11.18 ng/mL, corresponding to a specific activity is 8.94×10^4 Unit/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized CD320 at 25 ng/mL (100 μL/well) can bind Biotinylated TCN2. The ED₅₀ for this effect is 11.18 ng/mL, corresponding to a specific activity is 8.94×10⁴ Unit/mg.

Mouse

HEK293

C-6*His

O88968 (E19-W430)

21452

EFCVIPRIDSQLVEKLGQRLLPWMDRLSSEQLNPSVFVGLRLSSMQAGTKEDLYLHSLKIHYQQCLLRSTSSDDNSSCQPKLSGGSLALYLLALRANCEFFGSRKGDRLISQLKWFLEDEKKAIGHNHEGHPNTNYYQYGLSILALCVHQKRLHDSVVGKLLYAVEHDYFTYQGHVSVDTEAMAGLALTCLERFNFNSDLRPRITMAIETVREKILKSQAPEGYFGNIYSTPLALQMLMTSPASGVGLGTACIKAGTSLLLSLQDGAFQNPLMISQLLPILNHKTYLDLIFPDCQASRVMLVPAVEDPVHISEVISVTLKVASALSPYEQTFFVFAGSSLEDVLKLAQDGGGFTYGTQASLSGPYLTSVLGKDAGDREYWQLLRAPDTPLLQGIADYKPQDGETIELRLVRW

Approximately 42.4 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.