UBE2F Protein, Human (His)

UBE2F Protein, in the ubiquitin-like NEDD8 conjugation pathway, accepts NEDD8 from the UBA3-NAE1 E1 complex and covalently attaches it to various target proteins. Interacting specifically with the E3 ubiquitin ligase RBX2, not RBX1, the RBX2-UBE2F complex is specialized in neddylating specific targets, notably CUL5. This specific interaction and neddylating activity underscore UBE2F's role in regulating cellular processes through targeted ubiquitination of key substrates. UBE2F Protein, Human (His) is the recombinant human-derived UBE2F protein, expressed by E. coli, with N-His labeled tag. The total length of UBE2F Protein, Human (His) is 185 a.a., with molecular weight of ~25 kDa.

UBE2F protein plays a crucial role in the ubiquitin-like protein NEDD8 conjugation pathway, accepting NEDD8 from the UBA3-NAE1 E1 complex and catalyzing its covalent attachment to various target proteins. Its distinctive interaction with the E3 ubiquitin ligase RBX2, rather than RBX1, implies that the RBX2-UBE2F complex is specialized in neddylating specific target proteins, notably CUL5. This specific interaction and neddylating activity highlight UBE2F's involvement in the regulation of cellular processes related to the targeted ubiquitination of key substrates.

Human

E. coli

N-His

Q969M7 (M1-R185)

140739  [NCBI]

Approximately 25 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.