USF1 Protein, Human (His)

The USF1 protein is a transcription factor that binds symmetric E-box DNA (5'-CACGTG-3') in viral and cellular promoters. Efficient DNA binding requires dimerization, typically using bHLH proteins. USF1 Protein, Human (His) is the recombinant human-derived USF1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of USF1 Protein, Human (His) is 308 a.a., with molecular weight of 37.3 kDa.

The USF1 protein functions as a transcription factor, binding to symmetrical DNA sequences known as E-boxes (5'-CACGTG-3'), which are prevalent in a diverse array of viral and cellular promoters. Effective DNA binding necessitates dimerization, typically with another basic helix-loop-helix (bHLH) protein. USF1 engages in DNA binding both as a homodimer and as a heterodimer in association with USF2. Notably, USF1 also interacts with the varicella-zoster virus IE62 protein, indicating its involvement in molecular interactions with viral components. This versatile binding capacity and interaction profile highlight the regulatory role of USF1 in transcriptional processes across various cellular and viral contexts.

Human

E. coli

N-6*His

P22415 (G3-N310)

7391  [NCBI]

GQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

37.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.