2. Metabolic Enzyme/Protease MAPK/ERK Pathway Stem Cell/Wnt NF-κB Apoptosis
  3. Phosphatase ERK NF-κB Apoptosis
  4. Sanggenon C

Sanggenon C, a flavonoid, exerts protective effects against cardiac hypertrophy and fibrosis via suppression of the calcineurin/NFAT2 pathway. Sanggenon C inhibits mitochondrial fission to induce apoptosis by blocking the ERK signaling pathway. Sanggenon C inhibits inducible nitric oxide synthase expression in RAW264.7 cells, and TNF-α-stimulated cell adhesion and VCAM-1 expression, by suppressing NF-κB activity. Sanggenon C possesses antioxidant, anti-inflammatory and antitumor activities.

For research use only. We do not sell to patients.

Sanggenon C Chemical Structure

Sanggenon C Chemical Structure

CAS No. : 80651-76-9

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5 mg USD 240 In-stock
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Based on 4 publication(s) in Google Scholar

Sanggenon C Related Antibodies

Top Publications Citing Use of Products

    Sanggenon C purchased from MedChemExpress. Usage Cited in: J Nat Prod. 2022 Oct 18.  [Abstract]

    Sanggenon C (6, 8, 10 µM) induces an increase in apoptosis markers in HGC-27 and AGS cells.

    Sanggenon C purchased from MedChemExpress. Usage Cited in: J Nat Prod. 2022 Oct 18.  [Abstract]

    Sanggenon C (0, 6, 8, 10 µM) decreases the levels of CDK4 and cyclin D1 in HGC-27 cells.

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    • Biological Activity

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    Description

    Sanggenon C, a flavonoid, exerts protective effects against cardiac hypertrophy and fibrosis via suppression of the calcineurin/NFAT2 pathway. Sanggenon C inhibits mitochondrial fission to induce apoptosis by blocking the ERK signaling pathway. Sanggenon C inhibits inducible nitric oxide synthase expression in RAW264.7 cells, and TNF-α-stimulated cell adhesion and VCAM-1 expression, by suppressing NF-κB activity. Sanggenon C possesses antioxidant, anti-inflammatory and antitumor activities[1][2].

    IC50 & Target[1][2]

    NF-κB

     

    ERK

     

    In Vitro

    Sanggenon C (4-12 μM; 24 h) inhibits the proliferation of human gastric cancer (GC) cells and greatly reduces the number of GC cell colonies formed[2].
    Sanggenon C (6-10 μM; 24 h) inhibits cell cycle arrest and apoptosis of GC cells[2].
    Sanggenon C (6-10 μM; 24 h) markedly downregulates the levels of p-ERK[2].
    Sanggenon C (6-10 μM; 24 h) induces mitochondrial dysfunction of GC cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sanggenon C Related Antibodies

    Cell Proliferation Assay[2]

    Cell Line: Human GC cell lines HGC-27 and AGS cells
    Concentration: 4-12 μM
    Incubation Time: 24 h
    Result: Inhibited the proliferation of GC cells in a dose-dependent manner.
    The IC50 values of were 9.129 μM for HGC-27 and 9.863 μM for AGS.

    Cell Cycle Analysis[2]

    Cell Line: Human GC cell lines HGC-27 and AGS cells
    Concentration: 6, 8, 10 μM
    Incubation Time: 24 h
    Result: The proportions of cells in the G0-G1 phase were increased and the levels of CDK4 and cyclin D1 were decreased.

    Apoptosis Analysis[2]

    Cell Line: Human GC cell lines HGC-27 and AGS cells
    Concentration: 6, 8, 10 μM
    Incubation Time: 24 h
    Result: Exhibited a dose-dependent induction of apoptosis, with the percentage of apoptotic cells increasing from 7.3% to 24.8% and from 4.6% to 15.1% for HGC-27 and AGS cells, respectively.

    Western Blot Analysis[2]

    Cell Line: Human GC cell lines HGC-27 and AGS cells
    Concentration: 6, 8, 10 μM
    Incubation Time: 24 h
    Result: The levels of p-ERK were markedly downregulated.
    In Vivo

    Sanggenon C (10, 20 mg/kg/day; Intraperitoneally; for 3 weeks) improves impaired cardiac function following aortic banding (AB). Sanggenon C protects against cardiac hypertrophy[1].
    Sanggenon C (10, 20 mg/kg/day; Intraperitoneally; for 21 days) suppresses the tumor burden in the nude mice bearing tumor xenografts derived from AGS. Sanggenon C downregulates levels of p-ERK expression[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57/BL6 mice (weight 23.5-27.5 g; age, 8 weeks)[1]
    Dosage: 10, 20 mg/kg
    Administration: Intraperitoneally; daily; for 3 weeks
    Result: Prevented the development of ventricular dysfunction, as evidenced by decreased LV end-diastolic diameter, LV end-systolic diameter, and increased LVFS and LVEF.
    Molecular Weight

    708.71

    Formula

    C40H36O12
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to green yellow

    SMILES

    C/C(C)=C/C[C@](C1=CC=C(O)C=C1O2)(OC3=CC(O)=C([C@@H](C=C(C)C[C@@H]4C(C=CC(O)=C5)=C5O)[C@@H]4C(C(C=CC(O)=C6)=C6O)=O)C(O)=C3C7=O)[C@]27O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (141.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4110 mL 7.0551 mL 14.1101 mL
    5 mM 0.2822 mL 1.4110 mL 2.8220 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (3.53 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.50%

    COA (253 KB) HNMR (162 KB) CNMR (136 KB) RP-HPLC (215 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.4110 mL 7.0551 mL 14.1101 mL 35.2754 mL
    5 mM 0.2822 mL 1.4110 mL 2.8220 mL 7.0551 mL
    10 mM 0.1411 mL 0.7055 mL 1.4110 mL 3.5275 mL
    15 mM 0.0941 mL 0.4703 mL 0.9407 mL 2.3517 mL
    20 mM 0.0706 mL 0.3528 mL 0.7055 mL 1.7638 mL
    25 mM 0.0564 mL 0.2822 mL 0.5644 mL 1.4110 mL
    30 mM 0.0470 mL 0.2352 mL 0.4703 mL 1.1758 mL
    40 mM 0.0353 mL 0.1764 mL 0.3528 mL 0.8819 mL
    50 mM 0.0282 mL 0.1411 mL 0.2822 mL 0.7055 mL
    60 mM 0.0235 mL 0.1176 mL 0.2352 mL 0.5879 mL
    80 mM 0.0176 mL 0.0882 mL 0.1764 mL 0.4409 mL
    100 mM 0.0141 mL 0.0706 mL 0.1411 mL 0.3528 mL
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    Sanggenon C Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sanggenon C80651-76-9PhosphataseERKNF-κBApoptosisExtracellular signal regulated kinasesNuclear factor-κBNuclear factor-kappaBflavonoidcardiac hypertrophyfibrosiscalcineurinNFAT2mitochondrial fissioninducible nitric oxideRAW264.7TNF-αVCAM-1antioxidantanti-inflammatoryantitumorInhibitorinhibitorinhibit

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