2. Apoptosis
  3. Apoptosis
  4. Stevioside

Stevioside is an orally active sweetener that can be isolated from Stevia rebaudiana, with antihypertensive, antihyperglycemic, antioxidant, anti-inflammatory and anti-tumor activities.

For research use only. We do not sell to patients.

Stevioside Chemical Structure

Stevioside Chemical Structure

CAS No. : 57817-89-7

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10 mM * 1 mL in DMSO
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Customer Review

Based on 3 publication(s) in Google Scholar

Stevioside Related Antibodies

Top Publications Citing Use of Products

    Stevioside purchased from MedChemExpress. Usage Cited in: J Orthop Translat. 2023, 38: 190-202.

    Stevioside (STE; 10, 20, 40 µM) significantly decreases the expression of iNOS and COX-2 in a dose-dependent manner compared to stimulation with IL-1β.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Stevioside is an orally active sweetener that can be isolated from Stevia rebaudiana, with antihypertensive, antihyperglycemic, antioxidant, anti-inflammatory and anti-tumor activities[1][2].

    In Vitro

    Stevioside (10 μM, 24-48 h) can enhance cytotoxicity of 5-Fluorouracil (5-Fu, HY-90006) to MDA-MB-231 and SKBR3cells[1].
    Stevioside (10 μM, 48 h) combinated with 5-Fu (HY-90006) could increase the ratio of Bax to Bcl-2 in MDA-MB-231 cells, thereby triggering apoptosis[1].
    Stevioside (10-1000 μM, 6 h) induces TNF-α and IL-1β release in THP-1 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Stevioside Related Antibodies

    Apoptosis Analysis[1]

    Cell Line: MDA-MB-231
    Concentration: 10 μM
    Incubation Time: 24 h
    Result: Increased the rate of apoptosis in cells with combination of 5-Fu (HY-90006).
    Increased ROS level significantly in MDA-MB-231.
    Combinated with 5-Fu (HY-90006) could trigger early and late apoptotic events in cancer cells.

    Western Blot Analysis[2]

    Cell Line: THP-1 cells
    Concentration: 1 mM
    Incubation Time: 45 min
    Result: Inhibited the expression level of NF-κB and IKKβ.
    In Vivo

    Stevioside (20 mg/kg, p.o., daily, 10 days) prevents significant increase in glycaemia in NMRI Haan mice[3].
    Stevioside (50-200 mg/kg, i.v., one time) reduces blood pressure in a dose-dependent manner in spontaneously hypertensive rats[4].
    Stevioside (6.25-25 mg/kg, p.o., daily, 15 days) increases the proliferative response of in LPS stimulated B-lymphocytes in Balb/c mice[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Balb/c mice[5]
    Dosage: 6.25-25 mg/kg
    Administration: p.o., daily, 7 days
    Result: Didn’t show any change in the general behaviour of the test animals.
    Increased antibody synthesis of 15.38% and delayed type hypersensitivity (DTH) response of 18% at the dose of 12.5 mg/kg.
    Possessed macrophage stimulatory activity and increased phagocytic index.
    Clinical Trial
    Molecular Weight

    804.87

    Formula

    C38H60O18
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@]([C@]1([H])CC2)(CCC[C@@]1(C)C(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)=O)[C@@]4([H])[C@]2(CC5=C)C[C@@]5(O[C@@](O[C@H](CO)[C@@H](O)[C@@H]6O)([H])[C@@H]6O[C@]([C@@H]([C@@H](O)[C@@H]7O)O)([H])O[C@@H]7CO)CC4
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 250 mg/mL (310.61 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.2424 mL 6.2122 mL 12.4244 mL
    5 mM 0.2485 mL 1.2424 mL 2.4849 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (2.58 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (2.58 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.65%

    COA (265 KB) HNMR (355 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.2424 mL 6.2122 mL 12.4244 mL 31.0609 mL
    5 mM 0.2485 mL 1.2424 mL 2.4849 mL 6.2122 mL
    10 mM 0.1242 mL 0.6212 mL 1.2424 mL 3.1061 mL
    15 mM 0.0828 mL 0.4141 mL 0.8283 mL 2.0707 mL
    20 mM 0.0621 mL 0.3106 mL 0.6212 mL 1.5530 mL
    25 mM 0.0497 mL 0.2485 mL 0.4970 mL 1.2424 mL
    30 mM 0.0414 mL 0.2071 mL 0.4141 mL 1.0354 mL
    40 mM 0.0311 mL 0.1553 mL 0.3106 mL 0.7765 mL
    50 mM 0.0248 mL 0.1242 mL 0.2485 mL 0.6212 mL
    60 mM 0.0207 mL 0.1035 mL 0.2071 mL 0.5177 mL
    80 mM 0.0155 mL 0.0777 mL 0.1553 mL 0.3883 mL
    100 mM 0.0124 mL 0.0621 mL 0.1242 mL 0.3106 mL
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    Stevioside Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Stevioside57817-89-7Apoptosisantihypertensiveantihyperglycemicantioxidantanti-inflammatoryanti-tumorsweetenerchemosensitization5-FUInhibitorinhibitorinhibit

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