2. Immunology/Inflammation
  3. STING
  4. STING-IN-4

STING-IN-4 (Compound 1) is a STING inhibitor that inhibits STING expression and hence reducing activation of STING and nuclear factor-κB (NF-κB) signaling. STING-IN-4 shows anti-inflammatory activity and can be used for the research of sepsis.

For research use only. We do not sell to patients.

STING-IN-4 Chemical Structure

STING-IN-4 Chemical Structure

CAS No. : 2250374-27-5

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STING-IN-4 Related Antibodies

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Description

STING-IN-4 (Compound 1) is a STING inhibitor that inhibits STING expression and hence reducing activation of STING and nuclear factor-κB (NF-κB) signaling. STING-IN-4 shows anti-inflammatory activity and can be used for the research of sepsis[1].

IC50 & Target

STING[1]
In Vitro

STING-IN-4 (Compound 1) (20 μM; 26 h) inhibits NO production induced by LPS in RAW264.7 cells[1].
STING-IN-4 (2.5-10 μM; 26 h) significantly inhibits the expression of iNOS in RAW264.7 cells[1].
STING-IN-4 (5 and 50 μM; 12 h) significantly reduces STING degradation at 49, 52, and 55 °C and it may have an ability to interact with STING and enhance thermal stabilization of STING[1].
STING-IN-4 (2.5-10 μM; 8 h) inhibits LPS-induced activation of STING/IRF3/NF-κB[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

STING-IN-4 Related Antibodies

Western Blot Analysis[1]

Cell Line: RAW264.7 cells
Concentration: 2.5, 5 and 10 μM
Incubation Time: Pre-treated for 2 h followed LPS treatment for 6 h or 24 h
Result: Inhibited the expression of iNOS (24 h). Blocked LPS-induced phosphorylation of TBK1, IRF3, p65, and IκB-α (6 h).
In Vivo

STING-IN-4 (Compound 1) (1-9 mg/kg; i.p.; daily for 3 days) protects against LPS-induced liver injuries in mice and inhibits STING/IRF3/NF-κB activation in septic mice livers[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c mice, LPS-induced acute liver injury model[1]
Dosage: 1, 3 and 9 mg/kg
Administration: Intraperitoneal injection, daily for 3 days
Result: Significantly reduced the hemorrhage severity. Decreased the levels of alanine transaminase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) induced by LPS. Significantly reduced the levels of TNF-α, IL-6, and IFN-β compared with mice treated only with LPS. Markedly reduced the levels of STING, p-TBK, p-IRF3, p-p65, and p-IκB-α.
Molecular Weight

506.72

Formula

C32H46N2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]3([H])[C@]([C@]4(C([C@@]5([H])[C@@](CC4)(CCC(C)(C5)C)C(O)=O)=CC3)C)(CC[C@@]1([H])[C@@](C)(C6=NC=CN=C6C2)CO)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 93.33 mg/mL (184.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9735 mL 9.8674 mL 19.7348 mL
5 mM 0.3947 mL 1.9735 mL 3.9470 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.5 mg/mL (6.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 3.5 mg/mL (6.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (35.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9735 mL 9.8674 mL 19.7348 mL 49.3369 mL
5 mM 0.3947 mL 1.9735 mL 3.9470 mL 9.8674 mL
10 mM 0.1973 mL 0.9867 mL 1.9735 mL 4.9337 mL
15 mM 0.1316 mL 0.6578 mL 1.3157 mL 3.2891 mL
20 mM 0.0987 mL 0.4934 mL 0.9867 mL 2.4668 mL
25 mM 0.0789 mL 0.3947 mL 0.7894 mL 1.9735 mL
30 mM 0.0658 mL 0.3289 mL 0.6578 mL 1.6446 mL
40 mM 0.0493 mL 0.2467 mL 0.4934 mL 1.2334 mL
50 mM 0.0395 mL 0.1973 mL 0.3947 mL 0.9867 mL
60 mM 0.0329 mL 0.1645 mL 0.3289 mL 0.8223 mL
80 mM 0.0247 mL 0.1233 mL 0.2467 mL 0.6167 mL
100 mM 0.0197 mL 0.0987 mL 0.1973 mL 0.4934 mL
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STING-IN-4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

STING-IN-42250374-27-5STINGStimulator of Interferon GenesTMEM173MITAERISMPYSsepsisRAW264.7iNOSInhibitorinhibitorinhibit

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