2. Protein Tyrosine Kinase/RTK
  3. DYRK
  4. tBID

tBID is a selective inhibitor of homeodomain-interacting protein kinase 2 (HIPK2) with an IC50 of  0.33 µM.

For research use only. We do not sell to patients.

tBID Chemical Structure

tBID Chemical Structure

CAS No. : 1639895-85-4

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 489 In-stock
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10 mM * 1 mL in DMSO USD 489 In-stock
Solid
1 mg USD 180 In-stock
5 mg USD 420 In-stock
10 mg USD 600 In-stock
50 mg USD 1800 In-stock
100 mg USD 2520 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

tBID Related Antibodies

Top Publications Citing Use of Products

View All DYRK Isoform Specific Products:

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DYRK1 DYRK2 DYRK4

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

tBID is a selective inhibitor of homeodomain-interacting protein kinase 2 (HIPK2) with an IC50 of  0.33 µM.

IC50 & Target

IC50: 0.33 µM (HIPK2)[1]
In Vitro

Homeodomain-interacting protein kinase 2 (HIPK2) is a Ser/Thr kinase controlling cell proliferation and survival. TBID, displays toward HIPK2 unprecedented efficacy (IC50=0.33 µM) and selectivity (Gini coefficient 0.592 out of a panel of 76 kinases). The two other members of the HIPK family, HIPK1 and HIPK3, are also inhibited by TBID albeit less efficiently than HIPK2. The mode of action of TBID is competitive with respect to ATP, consistent with modelling. TBID interacts with the hinge region through hydrophobic interactions between Val 213, Val 261, Phe 277, Leu 280, Met 331, Ile 345, and the tetrabromine moiety, while the symmetric nitrogen atom at position 3 interacts with the catalytic Lys 228, thus playing a crucial role in the binding architecture[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

tBID Related Antibodies
Molecular Weight

528.78

Formula

C11H3Br4N3O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N(C2=NC=CN2)C(C3=C(Br)C(Br)=C(Br)C(Br)=C31)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (37.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8911 mL 9.4557 mL 18.9115 mL
5 mM 0.3782 mL 1.8911 mL 3.7823 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.03%

COA (254 KB) HNMR (441 KB) LCMS (130 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

HepG2 cells (human hepatocellular carcinoma) are cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 unit/mL penicillin and 100 µg/mL streptomycin. Treatments with TBID are performed in the same medium but with 1% fetal calf serum; control cells are treated with the solvent (DMSO). Cell viability is evaluated by means of MTT[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8911 mL 9.4557 mL 18.9115 mL 47.2786 mL
5 mM 0.3782 mL 1.8911 mL 3.7823 mL 9.4557 mL
10 mM 0.1891 mL 0.9456 mL 1.8911 mL 4.7279 mL
15 mM 0.1261 mL 0.6304 mL 1.2608 mL 3.1519 mL
20 mM 0.0946 mL 0.4728 mL 0.9456 mL 2.3639 mL
25 mM 0.0756 mL 0.3782 mL 0.7565 mL 1.8911 mL
30 mM 0.0630 mL 0.3152 mL 0.6304 mL 1.5760 mL
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tBID Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

tBID1639895-85-4DYRKDual specificity tyrosine phosphorylation regulated kinaseDual specificity tyrosine regulated kinaseInhibitorinhibitorinhibit

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