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Hoechst 33342 trihydrochloride Data Sheet
| Product Name: | Hoechst 33342 trihydrochloride |
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|---|---|---|
| CAS No.: | 875756-97-1 | |
| Cat. No.: | HY-15559A | |
| MWt: | 561.93 | |
| Formula: | C27H31Cl3N6O | |
| Purity : | >98% | |
| Solubility: | DMSO : ≥ 46 mg/mL;H<sub>2</sub>O : ≥ 5.6 mg/mL | |
| Mechanisms: | Target: Others | |
| Biological Activity: | ||
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Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain.
IC50 & Target: Dye reagent[1]
DNA Stain[1] In Vitro: Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1]. |
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