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  3. Hoechst 33342 trihydrochloride

Hoechst 33342 trihydrochloride  (Synonyms: bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride)

Cat. No.: HY-15559A Purity: 99.53%
COA Handling Instructions

Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain.

For research use only. We do not sell to patients.

Hoechst 33342 trihydrochloride Chemical Structure

Hoechst 33342 trihydrochloride Chemical Structure

CAS No. : 875756-97-1

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Customer Review

Based on 43 publication(s) in Google Scholar

Other Forms of Hoechst 33342 trihydrochloride:

Top Publications Citing Use of Products

33 Publications Citing Use of MCE Hoechst 33342 trihydrochloride

IF

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: Chinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.

    PC12 cells are treated with 1.5 mM Methylglyoxal (MG) for 24 h before pretreatment with Butein (2.5, 5 μM) for 1 h. Propidium iodide (PI) and Hoechst 33342 are used to determine cell apoptosis.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30081-30091.  [Abstract]

    Hoechst 33342 staining shows the survival of HUVECs administrated 100 μg/mL exosomes or CS-Exo upon exposure to 400 μM H2O2.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: Bioact Mater. 2022 Mar 17;18:91-103.  [Abstract]

    The fluorescent images of HUVECs stained with NO fluorescent probe (DAF-FM DA) and Hoechst 33342 after being treated with UCATS under dark conditions or 808 nm NIR irradiation.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: Chem Eng J. 15 December 2022, 138004.

    Apoptotic cells in four groups were stained with Hoechst 33342 (for 10 min) reflected by bright blue nuclei, including hP-MSCs cultured on noncoated, L-Gel-coated (10 nM), and D-Gel coated (10 nM) plates under exposure of 500 μM H2O2 for 5 h except for the normal group, and PBS as control.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: Theranostics. 2021 Mar 4;11(10):4655-4671.  [Abstract]

    Hoechst 33342 (10 µg/mL) is added to the medium of cultured megakaryocytes for 2 h at 37°C. Cells are stained with the directly coupled monoclonal antibodies PE-anti-CD41 and APC-anti-Mpl for 30 min at 4°C. Ploidy is measured in the CD41+Mpl+ cell population.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: Acta Biomater. 2020 Sep 1;113:289-304.  [Abstract]

    Hoechst 33342 staining (for 6 h) is used to distinguish apoptotic changes in H2O2-treated hP-MSCs.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: Aging Cell. 2021 Jul;20(7):e13382.  [Abstract]

    BM cells are incubated with 5 μg/mL Hoechst 33342 in Hank balanced salt solution containing 20 mM HEPES, 5 mM glucose, and 10% fetal bovine serum at 37°C for 45 min, followed by an additional 45 min of incubation with 1 μg/ml Pyronin Y.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: Stem Cell Res Ther. 2021 Jan 22;12(1):77.  [Abstract]

    To evaluate the anti-apoptosis property of extracellular vesicles from hP-MSCs, the Hoechst 33342 (for 10 min) is used to distinguish the apoptotic cells in the three different groups.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: J Cell Biol. 2021 Oct 4;220(10):e202011153.  [Abstract]

    Chromatin is briefly counterstained with Hoechst 33342 (5 µg/mL) before imaging.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: J Lipid Res. 2022 May;63(5):100207.  [Abstract]

    complete medium containing 1 μg/mL BODIPY 500/510 for 3 h and then 2 μg/mL Hoechst 33342 for an additional 2 min.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain.

    IC50 & Target

    Dye reagent[1]
    DNA Stain[1]

    In Vitro

    Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    561.93

    Formula

    C27H31Cl3N6O

    CAS No.
    Emission (Em)
    Excitation (Ex)
    SMILES

    [H]Cl.[H]Cl.[H]Cl.CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture and light

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 46 mg/mL (81.86 mM)

    H2O : ≥ 5.6 mg/mL (9.97 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7796 mL 8.8979 mL 17.7958 mL
    5 mM 0.3559 mL 1.7796 mL 3.5592 mL
    10 mM 0.1780 mL 0.8898 mL 1.7796 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 3.33 mg/mL (5.93 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.53%

    Dyeing Example
    References
    Cell Assay
    [1]

    Labeling Nuclear DNA with Hoechst 33342[1] Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+. Step 5, Mount the coverslips. Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Hoechst 33342 trihydrochloride
    Cat. No.:
    HY-15559A
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