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Hoechst 33342 trihydrochloride (Synonyms: bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride)

Cat. No.: HY-15559A Purity: 99.87%
Handling Instructions

Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain.

For research use only. We do not sell to patients.

Hoechst 33342 trihydrochloride Chemical Structure

Hoechst 33342 trihydrochloride Chemical Structure

CAS No. : 875756-97-1

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10 mg USD 60 In-stock
Estimated Time of Arrival: December 31
50 mg USD 120 In-stock
Estimated Time of Arrival: December 31
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Other Forms of Hoechst 33342 trihydrochloride:

Top Publications Citing Use of Products

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: Chinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.

    PC12 cells are treated with 1.5 mM Methylglyoxal (MG) for 24 h before pretreatment with Butein (2.5, 5 μM) for 1 h. Propidium iodide (PI) and Hoechst 33342 are used to determine cell apoptosis.

    Hoechst 33342 trihydrochloride purchased from MCE. Usage Cited in: ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30081-30091.

    Hoechst 33342 staining shows the survival of HUVECs administrated 100 μg/mL exosomes or CS-Exo upon exposure to 400 μM H2O2.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain.

    IC50 & Target

    Dye reagent[1]
    DNA Stain[1]

    In Vitro

    Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 46 mg/mL (81.86 mM)

    H2O : ≥ 5.6 mg/mL (9.97 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7796 mL 8.8979 mL 17.7958 mL
    5 mM 0.3559 mL 1.7796 mL 3.5592 mL
    10 mM 0.1780 mL 0.8898 mL 1.7796 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [1]

    Labeling Nuclear DNA with Hoechst 33342[1] Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+. Step 5, Mount the coverslips. Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    561.93

    Formula

    C₂₇H₃₁Cl₃N₆O

    CAS No.

    875756-97-1

    SMILES

    [H]Cl.[H]Cl.[H]Cl.CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1

    Storage
    Powder -20°C 3 years
      4°C 2 years

    *The compound is unstable in solutions, freshly prepared is recommended.

    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.87%

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    Product Name:
    Hoechst 33342 trihydrochloride
    Cat. No.:
    HY-15559A
    Quantity:

    Hoechst 33342 trihydrochloride

    Cat. No.: HY-15559A