Ni-NTA 6FF Prepacked Column

Cat. No.: HY-K0220: FAQs
Manual SDS

MCE Ni-NTA 6FF Prepacked Column is ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

Size	Price	Stock	Quantity
1 mL	USD 54	In-stock	Estimated Time of Arrival: December 31
5 mL	USD 193	In-stock	Estimated Time of Arrival: December 31

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Ni-NTA 6FF Prepacked Column Featured Recommendations:

Description
Storage
Components
Documentation

Description
& Advantages

Ni-NTA His-Tag Purification Agarose, a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites, enables high-yield, high-purity purification of polyhistidine-tagged proteins. Ni-NTA His-Tag Purification Agarose has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of chemicals and pH values. This makes Ni-NTA 6FF Prepacked Column ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

Characteristics
Composition	6% highly cross-linked agarose
Bead Diameter	45-165 μm
Binding Capacity	>>40 mg 6× His-tagged Protein/mL Settled Resin
Application	0.3 MPa, 3 bar
Recommended Dose	50% slurry in a 20% ethanol solution

Storage

Store at 4°C for two years.

Components

Cat. No.	Product Name	Package
HY-K0220-1 mL	Ni-NTA 6FF Prepacked Column	1 mL
HY-K0220-5 mL	Ni-NTA 6FF Prepacked Column	5 mL

Documentation

Manual (131 KB) SDS (131 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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