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  3. Protein Purification
  4. Agarose Beads
  5. Ni-NTA 6FF Prepacked Column

Ni-NTA 6FF Prepacked Column 

Cat. No.: HY-K0220
Manual SDS

MCE Ni-NTA 6FF Prepacked Column is ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

Ni-NTA 6FF Prepacked Column
Size Price Stock Quantity
1 mL USD 54 In-stock
5 mL USD 193 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

Ni-NTA His-Tag Purification Agarose, a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites, enables high-yield, high-purity purification of polyhistidine-tagged proteins. Ni-NTA His-Tag Purification Agarose has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of chemicals and pH values. This makes Ni-NTA 6FF Prepacked Column ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

Characteristics
Composition 6% highly cross-linked agarose
Bead Diameter 45-165 μm
Binding Capacity > 40 mg 6× His-tagged Protein/mL
Maximum Pressure 0.3 MPa, 3 bar
Storage Solution 50% slurry in a 20% ethanol solution
Storage

4°C, 2 years.

Components
Cat. No. Product Name Package
HY-K0220-1 mL Ni-NTA 6FF Prepacked Column 1 mL
HY-K0220-5 mL Ni-NTA 6FF Prepacked Column 5 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Ni-NTA 6FF Prepacked Column
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HY-K0220
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