Rhodamine Phalloidin
Based on 8 publication(s) in Google Scholar
MCE Rhodamine Phalloidin is Phalloidin conjugated to the fluorescent dye Tetramethylrhodamine. Phalloidin binds F-actins with high selectivity while Rhodamine provides stable and bright orange fluorescence.
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Storage :
The solutions should be stable for at least 6 months if store at -20°C.
Protecting from light, and avoid freeze/thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Phalloidin is a mushroom-derived toxin which can be used to specifically label F-actins (actin filaments) of the cytoskeleton. It binds to all variants of actin filaments in many different species of animals and plants. Typically, Phalloidin is used conjugated to a fluorescent dye. Phalloidin binds F-actins with high selectivity while Rhodamine provides stable and bright orange fluorescence.
Rhodamine Phalloidin is 1000× stock solution in DMSO which is convenient for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
Protocol
1. Prepare 1× Rhodamine Phalloidin working solution:
Add 1 μL of 1000× Rhodamine Phalloidin DMSO solution to 1 mL of PBS containing with 1% BSA.
Note: a. Different cell types might be stained differently. The concentration of AMCA Phalloidin working solution should be prepared accordingly.
b. 1% bovine serum albumin (BSA) is used to reduce nonspecific background staining.
2. Stain the cells:
2.1 Wash cells 2-3 times with PBS. Fix the cells in 3.7% methanol-free formaldehyde solution in PBS for 10-30 minutes at room temperature.
Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
2.2 Wash the fixed cells 2-3 times in PBS.
2.3 Add 0.1% Triton X-100 in PBS into fixed cells for 3-5 minutes to increase permeability. Wash the cells 2-3 times in PBS.
2.4 Add 100 μL/well (96-well plate) of AMCA Phalloidin working solution into the fixed cells, and stain the cells at room temperature for 20 to 90 minutes.
2.5 Rinse cells gently with PBS 2-3 times to remove excess Rhodamine Phalloidin.
2.6 Run fluorescence microscope or other equipments at desired Ex/Em wavelengths (AMCA Phalloidin, Ex/Em=344/432 nm).
Publications
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Journal Impact Factor
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Most Recent
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J NanobiotechnologyEngineered bone-targeting apoptotic vesicles as a minimally invasive nanotherapy for heterotopic ossification. [Abstract]2025 May 14;23(1):348. PMID: 40369573
Rhodamine Phalloidin purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 14;23(1):348. [Abstract]
Immunofluorescence images of osteoclast cytoskeleton stained with rhodamine phalloidin for 30 min in the dark.
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Rhodamine Phalloidin purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Sep 15.
Confocal fluorescence microscopy images showing cellular uptake of PSSZ@C6 in L929 (right) and BEAS-2B (left) cells. Nuclei were stained with DAPI; F-actin was stained with Phalloidin. Scale bar: 50 μm.
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Mater Today BioSLC7A11-independent disulfidptosis induced by a two-pronged delivery strategy for bladder cancer chemotherapy and cisplatin-resistance reverse. [Abstract]2025 Nov 13:35:102547. PMID: 41322137
Rhodamine Phalloidin purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Nov 13:35:102547. [Abstract]
Representative CLSM images of the cytoskeletal changes in T24/5637 cells. F-actin was stained with Phalloidin.
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J Med ChemDesign, Synthesis, and Evaluation of Novel ROCK Inhibitors for Glaucoma Treatment: Insights into In Vitro and In Vivo Efficacy and Safety. [Abstract]2025 May 22;68(10):10008-10030. PMID: 40340390
Rhodamine Phalloidin purchased from MedChemExpress. Usage Cited in: J Med Chem. 2025 May 22;68(10):10008-10030. [Abstract]
Disruption of actin stress fibers and focal adhesions by compounds Netarsudil, Ripasudil, D25, and R3 visualized by rhodamine phalloidin staining (1:500, 4 h, dark) in HTM cells.
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Am J PatholTGFβ1-Induced Fibrotic Responses of Conjunctival Fibroblasts through the Wnt/β-Catenin/CRYAB Signaling Pathway. [Abstract]2024 Sep;194(9):1764-1779. PMID: 38879081
Rhodamine Phalloidin purchased from MedChemExpress. Usage Cited in: Am J Pathol. 2024 Sep;194(9):1764-1779. [Abstract]
Fluorescence imaging and statistical analysis of F-actin labeled with Rhodamine Phalloidin in HConFs. Red, F-actin; blue, nuclei. ICG-001 reduces the fluorescence intensity and disrupts the organization of the F-actin cytoskeleton.
Storage
The solutions should be stable for at least 6 months if store at -20°C.
Protecting from light, and avoid freeze/thaw cycles.
Components
| Cat. No. | Product Name | Ex (nm) | Em (nm) | Size | Package |
|---|---|---|---|---|---|
| HY-K0903 | Rhodamine Phalloidin | 552.0 | 578.0 | 300 T | 30 μL |
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