VF 647A-Annexin V/PI Apoptosis Detection Kit 

Apoptosis is a form of programmed cell death. Based on the cellular changes during programmed cell death, apoptosis is often classified into early, middle, and later stages. The later stages of apoptosis are characterized by changes in nuclear morphology, chromatin condensation, nuclear envelope degradation, and DNA fragmentation.

Annexin V is a 35-36 kDa Ca²⁺ - dependent phospholipid-binding protein that has a high affinity for PS. In normal live cells, Phosphatidylserine (PS) is located on the inner side of the cell membrane. Upon initiation of apoptosis, PS translocates from the inner to the outer leaflet of the membrane and binds with Annexin V. Therefore, Annexin V serves as one of the sensitive indicator of early-stage apoptosis. Once labeled with a fluorescent probe, Annexin V can be used to detect the early-stage apoptosis using fluorescence microscopy or flow cytometry. Propidium Iodide (PI) is a cell-membrane impermeable dye that can stain late-stage apoptosis cells and necrosis cells with red fluorescence.

MCE VF 647A-Annexin V/PI Apoptosis Detection Kit can be utilized to detect the level of cell apoptosis. After co-staining with Annexin V (labeled with a far-red fluorescent probe (VF 647A)) and PI, normal cells are essentially non-fluorescent (Annexin V-/PI-), early-stage apoptosis cells show far-red fluorescence (Annexin V+/PI-), late-stage apoptosis cells and necrosis cells show red fluorescence (Annexin V+/PI+).

4℃, 2 year

Keep away from light, do not freeze.