Biotin Apoptosis Detection Kit (TUNEL) 

Apoptosis is a form of programmed cell death. Based on the cellular changes during programmed cell death, apoptosis is often classified into early, middle, and later stages. The later stages of apoptosis are characterized by changes in nuclear morphology, chromatin condensation, nuclear envelope degradation, and DNA fragmentation.

The principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) in detecting cell apoptosis is that during apoptosis, certain DNA endonucleases are activated, which cleave genomic DNA between nucleosomes. In normal or proliferating cells, there is almost no DNA breakage, thus 3'-OH formation is scarce, leading to minimal labeling. The exposed 3'-OH can be catalyzed by terminal deoxynucleotidyl transferase (TdT) to incorporate biotin-labeled dUTP, which can conjugate with horseradish peroxidase (HRP) -labeled streptavidin-HRP. Through DAB staining catalyzed by HRP, apoptotic cells can be detected, enabling cell apoptosis detection.

MCE Biotin Apoptosis Detection Kit (TUNEL) can be used to detect apoptosis in cultured cells, as well as in frozen or paraffin sections. It selectively detects apoptotic cells and distinguishes between apoptotic and necrotic cells. Due to the distinct patterns of DNA fragmentation, necrotic cells or non-apoptotic cells with broken DNA strands caused by irradiation and drug therapy will not be identified as apoptotic cells.

-20°C, 2 years

Keep away from light.

1. Please centrifuge the regent to the bottom of the tube before use.

2. As Sodium azide (NaN₃) inhibits HRP, the reaction system must not contain NaN₃.

3. This product is for R&D use only, not for drug, house hold, or other uses.

4. For your safety and health, please wear a lab coat and disposable gloves to operate.